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目的采用高效液相色谱法对不同产地的6批野马追药材中野马追内酯A,B和金丝桃苷进行了定性定量分析。方法野马追内酯A,B的测定采用Agilent Extend-C18(4.6 mm×150 mm,5μm)为分析柱,乙腈-水(27∶73)为流动相,流速为1.0 mL.min 1,等度洗脱,检测波长为220 nm,柱温为30℃。金丝桃苷的测定以SB-C18为分析柱,乙腈-1%醋酸水溶液(12∶88)为流动相,流速为1.0 mL.min 1,等度洗脱,检测波长为255 nm。结果野马追内酯A在0.02~1.88μg,野马追内酯B在0.44~4.44μg内与峰面积呈良好的线性关系。金丝桃苷在0.101~1.414μg内与峰面积呈良好的线性关系。结论本实验方法准确、快速、灵敏、重复性好,在该色谱条件及样品处理方法下,可以准确测定野马追中野马追内酯A,B和金丝桃苷的含量。
Objective To establish a qualitative and quantitative analysis of Mustangolide A, B and Hyperoside in six batches of Mustang chasing herbs from different areas by high performance liquid chromatography (HPLC). Methods The determination of Mustangolide A and B was carried out on an Agilent Extend-C18 (4.6 mm × 150 mm, 5 μm) column with acetonitrile-water (27:73) as the mobile phase at a flow rate of 1.0 mL · min 1, Elution, detection wavelength was 220 nm, the column temperature was 30 ℃. The determination of hyperoside with SB-C18 as analytical column, acetonitrile-1% acetic acid aqueous solution (12:88) as the mobile phase, the flow rate of 1.0 mL.min 1, isocratic elution, detection wavelength of 255 nm. Results Wild horse chase lactone A in 0.02 ~ 1.88μg, wild horse chalcone B in 0.44 ~ 4.44μg and peak area showed a good linear relationship. Hyperin in 0.101 ~ 1.414μg within the peak area showed a good linear relationship. Conclusion The experimental method is accurate, rapid, sensitive and reproducible. Under the chromatographic conditions and sample processing methods, the contents of Mustangolide A, B and hyperoside in the Mustang can be accurately determined.