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磷是植物体内重要的营养元素.土壤中含磷总量丰富,但能被植物直接吸收利用的可溶性磷含量却很低,这成为制约农作物产量的重要因素.本研究利用水稻寡核苷酸芯片分析了水稻根部在正常营养条件和低磷胁迫6,24,72h3个时间点的全基因组表达谱.和正常营养条件下相比,低磷胁迫水稻根部共发现795个差异表达基因.差异表达基因功能分析发现:(1)磷酸盐转运蛋白、酸性磷酸酶、RNA酶等基因上升表达;(2)糖酵解等与能量代谢相关基因先上升后下降表达;(3)氮吸收和脂代谢相关基因改变其表达;(4)蛋白质降解、细胞衰老相关基因上升表达;(5)部分跨膜转运蛋白基因表达上调.研究结果为进一步揭示植物低磷胁迫反应机制,改善作物对磷吸收利用效率提供了有用的信息.
Phosphorus is an important nutritional element in plants, and the content of total phosphorus in soil is rich, but the content of soluble phosphorus that can be directly absorbed by plants is very low, which becomes an important factor restricting the yield of crops.In this study, using rice oligonucleotide chip The genome-wide expression profiles of rice roots at 6, 24, and 72 h at normal and low-P stress were analyzed.A total of 795 differentially expressed genes were found in roots of low-phosphorus-stressed rice roots under normal nutrition conditions.The differentially expressed genes The functional analysis showed that: (1) genes such as phosphate transporter, acid phosphatase and RNase were up-regulated; (2) genes related to energy metabolism, such as glycolysis, first increased and then decreased; (3) (5) Partial transmembrane transporter gene expression up-regulated.Results In order to further reveal the mechanism of plant low-phosphorus stress response and improve the efficiency of crop phosphorus uptake and utilization Useful information