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目的从微小核糖核酸(micro RNAs,mi RNAs)角度研究石荠苧总黄酮抗流感病毒性肺炎作用机制。方法设立对照组、模型组和石荠苧总黄酮组,将甲型流感病毒小鼠肺适应株A/PR/8/34经鼻滴入模型组和石荠苧总黄酮组小鼠,建立小鼠流感病毒感染性肺炎模型,观察比较石荠苧总黄酮对流感病毒感染小鼠肺指数和血清白细胞介素-6(IL-6)、γ干扰素(IFN-γ)水平的影响;采用高通量测序法和荧光定量PCR法,检测各组小鼠肺组织中mi RNAs的表达丰度差异;采用miranda、mirbase、targetscan 3个数据库预测差异mi RNAs的靶基因,并通过KEGG分析靶基因的相关功能;采用蛋白印迹法验证相关蛋白的表达。结果与模型组相比,石荠苧总黄酮可显著抑制流感病毒感染引起的小鼠肺指数和血清中细胞因子增加,调节异常表达的mi RNAs水平趋于正常;KEGG分析这些mi RNAs所调控的靶基因主要富集于JAK-STAT、TLR3等信号通路;Western blotting证实石荠苧总黄酮可调整感染小鼠异常表达的靶蛋白水平。结论石荠苧总黄酮可通过调控小鼠体内mi RNAs的表达发挥抗流感病毒性肺炎作用。
Objective To study the anti-influenza virus pneumonia mechanism of total flavones of Mosla henryi from microRNAs (mi RNAs). Methods A control group, a model group and a total flavone group were established. The lung-adapted strain A / PR / 8/34 of influenza A virus was dripped into the model group and the total flavone group To investigate the effects of total flavonoids of Mosla henryi on influenza virus-infected mice lung index and levels of serum interleukin-6 (IL-6) and interferon-gamma (IFN-γ) The expression abundance of mi RNAs in the lungs of mice was detected by flow cytometry and real-time quantitative PCR. Three miRNAs target genes were predicted by miranda, mirbase and targetscan, and the target genes were analyzed by KEGG Related functions; using Western blot to verify the expression of related proteins. Results Compared with the model group, total flavone of Mosla henryi could significantly inhibit the increase of lung index and serum cytokines in mice induced by influenza virus infection, and the normal miRNAs that regulate abnormal expression tended to be normal. KEGG analysis of these miRNAs regulated The target genes were mainly enriched in JAK-STAT, TLR3 and other signaling pathways; Western blotting confirmed that the total flavonoids of Mosla henryi could regulate the target protein levels abnormally expressed in infected mice. Conclusion The total flavones of Mosla henryi can play a role in anti-influenza virus pneumonia by regulating the expression of mi RNAs in mice.