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在丝虫病的免疫诊断方面,过去做了不少研究工作,但都是采用犬恶丝虫制备的抗原,而这种抗原敏感性和特异性都比较差。近来大沙鼠能保存马来丝虫的发现,为制备抗原提供了足够数量的人体丝虫的来源。[抗原的制备]微丝蚴抗原:用300条马来丝虫第3期(感染性)幼虫接种大沙鼠,4个月后用戊巴比妥纳麻醉,腹部剃毛,用18号血管导管插入腹腔中部以15毫升灭菌磷酸缓冲盐水洗涤腹腔2次,除去白细胞,先后
In the immunodiagnosis of filariasis, a lot of research work has been done in the past, but all of them are antigens prepared by Dirofilaria cannabis, and the sensitivity and specificity of this antigen are relatively poor. Recently, large gerbils can save the discovery of malayian filariasis and provide a sufficient source of human filarial worms for the preparation of antigens. [Preparation of Antigen] Filamentous Filariae Antigen: Ophiopogon japonicus was inoculated with 300 malayian worm stage 3 (infective) larvae, anesthetized with pentobarbitone 4 months later, and the abdomen shaved. A blood vessel of No. 18 Catheter inserted into the middle of the abdominal cavity to 15 ml sterilized phosphate buffered saline to wash the abdominal cavity 2 times to remove leukocytes, successively