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由于ＨＰＶ１６Ｅ６蛋白能诱导机体保护性免疫反应，可作为基因治疗的靶抗原。用杆状病毒昆虫细胞表达系统制备了ＨＰＶ１６Ｅ６基因工程蛋白，拟用于宫颈癌细胞系小鼠模型抗癌的免疫治疗。用ＰＣＲ技术从ＨＰＶ１６基因组中扩增获得转化基因Ｅ６的完整ＯＲＦ，按ＴＡ策略将其克隆到自行制备的杆状病毒转移载体ｐＶＬ１３９３Ｔ尾载体中，置于杆状病毒ＡｃＭＮＰＶＰｏｌｈ晚期启动子控制之下，用此重组转移质粒ｐＶＬ１３９３Ｅ６与杆状病毒ＤＮＡ共转染昆虫细胞Ｓｆ９，经噬斑筛选获得带有编码Ｅ６蛋白基因的重组杆状病毒株，并在昆虫细胞Ｓｆ９中表达为非融合性Ｅ６蛋白。ＳＤＳＰＡＧＥ电泳分析其分子量约为１８ｋＤ，免疫印迹实验表明，此重组蛋白能被兔抗ＨＰＶ１６Ｅ６抗体所识别。
Because HPV16E6 protein can induce protective immune response, it can be used as target antigen for gene therapy. The baculovirus-insect cell expression system was used to prepare the HPV16E6 gene engineering protein, which is intended for anti-cancer immunotherapy in mouse cervical cancer cell line. PCR technique was used to amplify the complete ORF of transformed E6 from HPV16 genome and cloned into the self-prepared baculovirus transfer vector pVL1393T-tail vector according to TA strategy and placed in the baculovirus AcMNPVPolh late promoter control Under this recombinant transfer plasmid pVL1393 E6 and baculovirus DNA co-transfected insect cells Sf 9, plaque screening with recombinant E6 protein gene encoding recombinant baculovirus strain, and insect cells Sf 9 Expressed as a non-fusion E6 protein. SDS-PAGE analysis of its molecular weight of about 18kD, Western blotting experiments show that the recombinant protein can be rabbit anti-HPV16E6 antibody recognition.