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关于血液流变学和肿瘤转移之间关系的研究国内外已有不少报道,但多数作者是从临床病人研究中发现血液粘度与恶性肿瘤间的相关性的。我们为了研究肿瘤在机体内侵袭和转移的整个发展过程中血液流变学的变化规律性,以及根据血液流变学异常变化程度而反应出的肿瘤侵袭和转移的进程,为临床提供血液流变学诊断依据,特设计本实验。实验用病理室建立的具有淋巴道合并血道双行转移的小鼠子宫颈癌14号(U14)。取接种后6～7天的5×10~6腹水细胞接种于近交系615小鼠的背侧部皮下。分4组,分别于接种后5、9、15和19天各处死14只动物,切取原发瘤及其周围组织、局部淋巴结和肺,用组织学方法确定侵袭和转移的程度,同时取血测定血液流变学的血浆粘度(η_b)、红细胞压积(H)、红细胞聚集指数和全血粘度值之比(η_(be)/η_(bh)。此外先后两批各处死10只正常小鼠测定血液流变学的正常指标作对照。
Many studies have been published on the relationship between hemorheology and tumor metastasis in China and abroad. However, most authors found the correlation between blood viscosity and malignant tumors from clinical patient studies. In order to study the regularity of hemorheology during the whole development of tumor invasion and metastasis in vivo and the progress of tumor invasion and metastasis according to the degree of abnormal change of hemorheology, Based on the diagnosis, special design of the experiment. The experiment was performed in a pathology laboratory with mouse uterine cervix carcinoma No. 14 (U14) with lymphatic and hematogenous double line metastasis. 5 × 10 ~ 6 ascites cells from 6 to 7 days after inoculation were subcutaneously inoculated into the dorsal part of 615 inbred mice. Divided into 4 groups, 14 animals were sacrificed at 5, 9, 15 and 19 days after inoculation, the primary tumor and its surrounding tissues, the local lymph nodes and the lungs were excised, the degree of invasion and metastasis was determined by histological method, and blood The blood viscosity (η_b), hematocrit (H), erythrocyte aggregation index and whole blood viscosity ratio (η be / η bh) were measured in the blood rheology.Furthermore, Mice were tested for normal indices of hemorheology as a control.