采用醋酸扭体法,对45种蕈类134种提取物的抗伤害感受作用进行了筛选,其中灵芝(Ganoderma lucidum)的二氯甲烷提取物呈现明显的镇痛作用,抑制率为35.6％。根据生物活性先导分离法,从灵芝的二氯甲烷提取物中分离得到5种活性成分。 实验方法如下:取风干的灵芝,用二氯甲烷室温提取3次(2小时),二氯甲烷提取液经硅胶柱层析,以正己烷丙酮(20:1～0:1) Using acetic acid writhing method, the antinociceptive effect of 134 kinds of extracts of 45 ticks were screened, among which the extract of Ganoderma lucidum showed obvious analgesic effect with inhibition rate of 35.6%. According to the biological activity pilot separation method, 5 active ingredients were isolated from the methylene chloride extract of Ganoderma lucidum. The experimental method is as follows: air-dried Ganoderma lucidum was extracted with dichloromethane three times at room temperature (2 hours), and the dichloromethane extract was chromatographed on a silica gel column with n-hexane acetone (20:1 to 0:1).