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Objective To provide the scientific evidence for the exploiture of aloe resource. Methods Cytological combined determination was used to study the effect of aloe glue(0.01 ～ 0.3ml) on cytogenetics, cellular immunity and cell proliferation of human cells. Results SCE and MNR in varying dose groups had no significant differences as compared with control group( P ＞ 0.05). LTR was significantly higher than that of control group(P ＜ 0.005). MI was significantly higher than that of control group ( P ＜ 0.05). M3 and PRI in highest dose group had significant differences as compared with control group (P ＜ 0.05). Conclusion Aloe gel had no significant effect on cytogenetics. But it had activating effects on immunity and proliferation of cells.