论文部分内容阅读
以ＥｃｏＲＩ酶切安徽地区卫氏并殖吸虫基因组ＤＮＡ，电泳分离回收０．４～４ｋｂ的ＤＮＡ片段。与ＥｃｏＲＩ酶切并经牛肠碱性磷酸脂酶处理的大肠杆菌质粒ｐＵＲ２２２载体，在Ｔ４ＤＮＡ连接酶作用下重组连接，构建卫氏并殖吸虫基因组ＤＮＡ文库，获得１．０８×１０６个重组克隆。经重组克隆质粒的快速鉴定和卫氏并殖吸虫基因组ＤＮＡ探针菌落原位杂交证实，重组克隆中含有与卫氏并殖吸虫基因组ＤＮＡ同源的ＤＮＡ插入片段，并初筛到１９个阳性重组克隆。表明卫氏并殖吸虫基因组ＤＮＡ文库构建成功。
The genomic DNA of Paragonimus westermani in Anhui area was digested with EcoRI, and the DNA fragment of 0.4-4 kb was isolated and recovered by electrophoresis. E. coli plasmid pUR222 vector digested with EcoRI and digested with alkaline phosphatase of calf intestine was recombined with T4 DNA ligase to construct a genomic DNA library of Paragonimus westermani and obtain 1.08 × 10 6 recombinant clones. The rapid identification of the recombinant cloning plasmid and Paragonimus westermani genomic DNA probe colony in situ hybridization confirmed that the recombinant cloning genomic DNA containing homologous Paragonimus westermani DNA inserts and screening to 19 positive recombination clone. The genomic DNA library of Paragonimus westermani was constructed successfully.