利用高效降解纤维素的菌株D1对水葫芦进行预处理,将其内部结构破坏后与活性污泥混合,在不添加任何底物情况下,对比经过预处理与未经预处理水葫芦厌氧发酵产沼气的优化条件,得出最佳优化条件。进一步将水葫芦厌氧发酵产沼气的最佳优化条件进行放大试验,结果表明:在10 L厌氧发酵罐内,100 g的水葫芦经5 mL含有107孢子的D1菌株菌悬液预处理48 h后,与厌氧活性污泥在30℃厌氧发酵60 d左右,其产气效率明显优于未经预处理的水葫芦,达15.52 L,平均产气量达155.2 mL/g,甲烷含量为63.5%。同时考察了反应器内的pH变化,结果表明pH值相对比较稳定,适合甲烷菌产气。 Water hyacinth was pretreated with strain D1, which degraded cellulose efficiently, and then mixed with activated sludge after its internal structure was destroyed. Without adding any substrate, the water hyacinth was compared with the anaerobic fermentation without water pretreatment Biogas production optimization conditions, come to the best optimization conditions. The optimum conditions of biogas production by anaerobic fermentation of water hyacinth were further studied. The results showed that 100 g of water hyacinth in 10 L anaerobic fermenter was pretreated with 5 mL of bacterial suspension of D1 strain with 107 spores h, the anaerobic sludge was anaerobically fermented at 30 ℃ for about 60 days and its gas production efficiency was obviously better than that of the untreated water hyacinth, reaching 15.52 L, with an average gas production of 155.2 mL / g and a methane content of 63.5%. At the same time, the pH change in the reactor was investigated. The results showed that the pH value was relatively stable and suitable for methane gas production.