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目的 探讨大鼠变应性鼻炎模型上、下呼吸道炎症反应的相关性及其机制。方法 选健康大鼠60只,随机分为变应性鼻炎组和对照组,每组30只。以卵清蛋白+Al(OH)3 基础致敏、局部以2%卵清蛋白激发制成大鼠变应性鼻炎模型。取实验动物鼻黏膜及肺组织做HE染色、阿森过碘酸雪夫(alcin blueandperiodicacid schiff, AB PAS)染色,分别观察炎症细胞及杯状细胞;透射电镜观察支气管黏膜上皮变化;以酶联免疫吸附测定法(enzyme linkedimmunosorbentassay, ELISA)检测支气管肺泡灌洗液(bronchialalveoluslavagefluid, BALF)中白细胞介素4 -(interleukin 4, IL- 4 )的水平;以免疫组化方法检测肺组织细胞间黏附分子1 (intercellularadhesionmolecule 1, ICAM -1)及鼻黏膜和肺组织黏蛋白5AC(Mucin5AC,MUC5AC)的表达。结果 大鼠变应性鼻炎模型组鼻黏膜及肺组织有明显的炎症细胞浸润,杯状细胞增生活跃并呈分泌旺盛状态,电镜下支气管黏膜部分纤毛破坏。模型组BALF中IL 4含量( x±s,下同)为(58 .10±7 .92)pg/ml,对照组为(24. 66±2 .07)pg/ml,两组比较差异有统计学意义(t=7. 06, P<0.05),且模型组肺组织ICAM 1及MUC5AC的表达(分别为0. 66±0. 24、0 .71±0 .10)与对照组(分别为0 .23±0 .02、0 .29±0. 03)比较,差异均有统计?
Objective To investigate the correlation and mechanism of inflammatory reaction in upper and lower respiratory tract of allergic rhinitis model in rats. Methods Sixty healthy rats were randomly divided into allergic rhinitis group and control group, with 30 rats in each group. Ovalbumin + Al (OH) 3 basal sensitization, local 2% ovalbumin-induced rat allergic rhinitis model. The nasal mucosa and lung tissues of experimental animals were stained with hematoxylin-eosin (HE) and stained with acinar acid and periodic acid (ABPAS), respectively. Inflammatory cells and goblet cells were observed respectively. The bronchial mucosal epithelium was observed by transmission electron microscopy and enzyme-linked immunosorbent assay (ELISA) was used to detect the level of interleukin 4 (IL-4) in bronchoalveolar lavage fluid (BALF). The expression of intercellular adhesion molecule 1 (IL-4) in lung tissue was detected by immunohistochemistry 1, ICAM-1) and the expression of mucins 5AC (Mucin5AC, MUC5AC) in nasal mucosa and lung tissue. Results In the rat model of allergic rhinitis, inflammatory cell infiltration was found in the nasal mucosa and lung tissue. The goblet cells proliferated actively and secreted exponentially. The bronchial mucosa partially ciliated damaged by electron microscopy. The level of IL-4 in BALF was (58.10 ± 7.92) pg / ml in model group and (24.66 ± 2.07) pg / ml in control group (T = 7.06, P <0.05). The expression of ICAM 1 and MUC5AC in the model group was significantly lower than that in the control group (0.66 ± 0.24,0.71 ± 0.10, respectively 0 .23 ± 0 .02,0 .29 ± 0. 03), the differences are statistically?