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目的 探讨黏附分子CD4 4、上皮性钙黏附蛋白 (E cad)和转移抑制基因nm2 3 H1与甲状腺滤泡源性癌分化、浸润转移的关系。方法 采用免疫组织化学SP法和EnVision法检测 4 2例滤泡癌和 5 4例乳头状癌中CD4 4、E cad和nm2 3 H1的表达。结果 CD4 4主要表达于癌细胞及浸润淋巴细胞胞膜 ,低分化滤泡癌和有转移乳头状癌CD4 4表达分别高于高分化滤泡癌和无转移乳头状癌。E cad阳性物质和nm2 3 H1基因产物均分布于癌细胞胞质 ,表达程度与滤泡癌分化呈正相关 ,乳头状癌的E cad和nm2 3 H1阳性率高于滤泡癌 ,转移癌的阳性率和表达强度低于原发灶。甲状腺滤泡源性癌CD4 4检测阳性率高于E cad和nm2 3 H1。在滤泡癌中E cad与nm2 3 H1的表达之间呈正相关关系 ,而在乳头状癌中呈正相关趋势。CD4 4与E cad的表达、CD4 4与nm2 3 H1的表达之间在滤泡癌和乳头状癌均呈负相关趋势。结论 综合检测CD4 4、E cad和nm2 3对甲状腺滤泡源性癌的诊断、预后评估具有一定参考价值
Objective To investigate the relationship between the expression of adhesion molecule CD4 4, E cad and metastasis suppressor gene nm2 3 H1 in follicular thyroid carcinoma. Methods Immunohistochemical SP method and EnVision method were used to detect the expression of CD4 4, E cad and nm23 H1 in 42 cases of follicular carcinoma and 54 cases of papillary carcinoma. Results CD4 4 was mainly expressed on the membrane of infiltrating lymphocytes and infiltrating lymphocytes. The expression of CD4 4 in poorly differentiated follicular carcinoma and metastatic papillary carcinoma was higher than that in highly differentiated follicular carcinoma and non-metastatic papillary carcinoma, respectively. E cad positive substance and nm23 H1 gene product were all distributed in the cytoplasm of cancer cells, the expression level was positively correlated with the differentiation of follicular carcinoma. The positive rates of E cad and nm23 H1 in papillary carcinoma were higher than those in follicular carcinoma and metastatic carcinoma Rate and expression intensity is lower than primary tumor. The positive rate of CD4 4 in thyroid follicular carcinoma was higher than that of E cad and nm23 H1. There was a positive correlation between E cad and the expression of nm23 H1 in follicular carcinoma, but positively correlated with papillary carcinoma. CD4 4 and E cad expression, CD4 4 and nm23 H1 expression in follicular and papillary carcinoma showed a negative correlation trend. Conclusion The combined detection of CD4 4, E cad and nm23 on thyroid follicular carcinoma of the diagnosis, prognosis evaluation has some reference value