论文部分内容阅读
光棘球海胆(Strongylocentrotus nudus)又称大连紫海胆,是我国主要的经济型海胆之一,其味道鲜美、营养丰富,具有较高的食用和药用价值。本实验以大连紫海胆为研究对象,采用酶解法制备海胆活性肽,并对其部分生物活性进行研究。使用木瓜蛋白酶对海胆黄进行水解,得到分子质量分布范围<1、1~3、3~5、5~10 k D和<10 k D的5种海胆多肽组分。活性研究结果显示,在多肽终质量浓度为200μg/m L时,不同分子质量分布的海胆多肽均可促淋巴细胞生长。在这一质量浓度下,除了小于1 k D的多肽,其他多肽均对人宫颈癌细胞(He La细胞)增殖有一定抑制作用,对腹腔巨噬细胞吞噬能力有一定促进作用。在多肽终质量浓度为50μg/m L时,不同组分海胆多肽对叔丁基脂氢过氧化物所致的Chang肝细胞氧化损伤均可发挥直接和间接抗氧化保护作用。然而,不同分子质量分布的海胆多肽均可抑制补体的经典途径活性,且呈现明显剂量-效应关系。上述结果表明,海胆多肽对细胞免疫和体液免疫功能具有潜在的调节作用,其功能活性的多向性值得深入研究。
Strongylocentrotus nudus, also known as Dalian purple sea urchin, is one of the major economic sea urchins in China. It is delicious and nutritious, and has high edible and medicinal value. In this experiment, purple sea urchin was taken as the research object, and the active peptide of sea urchin was prepared by enzymolysis, and some of its biological activities were studied. The sea urchin yellow was hydrolyzed by papain, and five kinds of sea urchin polypeptide components with molecular mass distribution range of <1,1-3, 3-5, 5-10 kD and <10 kD were obtained. The results of the activity study showed that when the final concentration of the polypeptide was 200 μg / mL, the different molecular weight distribution of E. coli could promote the growth of lymphocytes. At this mass concentration, except for polypeptides less than 1 kD, all other peptides inhibited the proliferation of human cervical cancer cells (HeLa cells) and promoted the phagocytosis of peritoneal macrophages. At the final peptide concentration of 50μg / mL, the direct and indirect antioxidative protection of different components of sea urchin peptides on the oxidative damage of Chang hepatocytes induced by tert-butyl hydroperoxide can be exerted. However, the sea urchin polypeptides with different molecular weight distributions could inhibit the classical pathway activity of complement and showed obvious dose-effect relationship. The above results show that the sea urchin polypeptide has a potential regulatory effect on cellular immunity and humoral immunity, and its multifunctional nature should be further studied.