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目的:研究高良姜、草豆蔻、红豆蔻、大高良姜、建砂仁、益智6味山姜属中药对胃实寒证模型大鼠胃组织磷酸二酯酶(PDE)、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、cAMP/cGMP的影响,揭示6味山姜属中药所含挥发油与温中散寒功效的相关性。方法:实验分2批进行,第1批取SD雄性大鼠160只分为16个组:模型Ⅰ组,模型Ⅱ组,阳性对照组,高良姜、草豆蔻、红豆蔻3味药物各个挥发油高、低剂量组、去挥发油水液高、低剂量组;第2批除了观察药为大高良姜、建砂仁、益智挥发油高、低剂量组、去挥发油水液高、低剂量组外,其余分组均同第1批。采用灌服2~3℃寒凉药(石膏、知母、黄柏、龙胆草,给药剂量为36g·kg-1,2次/d)加冷冻的方法制造大鼠胃实寒证模型;分别予以6味药物挥发油、去挥发油水液,低剂量组给药剂量为20g·kg-1,高剂量组给药剂量为60 g·kg-1,阳性对照药选用附子水煎液,剂量为8 g·kg-1;每隔12 h给药1次,连续给药3次。测定大鼠胃组织PDE,cAMP,cGMP含量,计算cAMP/cGMP。结果:①对磷酸二酯酶(PDE)的影响:与正常组相比,第1,2批模型组胃组织中PDE明显升高(P<0.05或P<0.01),分别为32.27,52.80 nmol·L-1。高良姜、草豆蔻、红豆蔻、大高良姜、益智的挥发油高、低剂量组对于模型组大鼠胃中PDE有显著的降低作用(P<0.05或P<0.01),大高良姜去挥发油高剂量组对其也有一定的降低作用,但无显著性差异;②对cAMP,cGMP及cAMP/cGMP的影响:与空白组比较,第1,2批模型组胃组织中cAMP的浓度明显降低(P<0.05),分别为0.68,0.81 nmol·L-1。6味中药挥发油高、低剂量组与模型组相比,cAMP浓度均明显升高(P<0.05或P<0.01),cGMP无明显变化,cAMP/cGMP增大(P<0.05或P<0.01);高良姜、草豆蔻、建砂仁去挥发油高剂量组对cAMP浓度也有一定的影响,但无显著性差异。结论:6味中药所含挥发油可升高胃组织中cAMP的含量、cAMP/cGMP及降低PDE的含量,其中部分中药的去挥发油高剂量组也有相似的影响,揭示了6味山姜属中药所含的挥发油与温中散寒功效有密切的相关性,且发现此6味药的去挥发油提取液对部分检测指标也有良好的改善作用,揭示其功效也与其所含的其他成分有关。
Objective: To study the effects of galangal, nutmeg, red cardamom, galangal, villosum and yogurt 6 Chinese medicine on gastric mucosal phosphodiesterase (PDE), cyclic adenosine monophosphate (cAMP), cGMP and cAMP / cGMP in order to reveal the correlation between volatile oil contained in traditional Chinese medicine of Six-flavored Ginger root and the effects of cold and warm in cold. Methods: The experiment was divided into 2 batches. The first batch of 160 male SD rats were divided into 16 groups: Model Ⅰ, model Ⅱ, positive control group, galangal, cardamom, red cardamom volatile oil , Low-dose group, high-dose and low-dose group of volatile oil and water; in the second batch, besides observing the drug of galangal, villosum, high and low dose of volatile oil, The remaining groups are the first batch. The gastric cold syndrome model of rats was made by freezing and cooling the cold and cool medicine (gypsum, Anemarrhena, Cortex phellodendri, gentian, the dosage was 36g · kg-1, 2 times / d) The volatile oil and volatile oil were respectively administrated to the low dose group (20g · kg-1) and the high dose group (60g · kg-1). The dosage of aconite decoction was 8 g · kg -1; dosing once every 12 h, continuous dosing 3 times. The content of PDE, cAMP and cGMP in gastric tissue of rats were measured to calculate cAMP / cGMP. Results: (1) The effect on phosphodiesterase (PDE): Compared with the normal group, the PDE in the first and the first batch of model group were significantly increased (P <0.05 or P <0.01), which were 32.27 and 52.80 nmol · L-1. Galangal, cardamom, cardamom, galangal, volatile oil of high and low dose of volatile oil had a significant effect on reducing PDE in model rats (P <0.05 or P <0.01) High-dose group also reduced its effect, but no significant difference; ② cAMP, cGMP and cAMP / cGMP impact: Compared with the blank group, the first and the first batch of model group of gastric tissue cAMP concentration was significantly reduced P <0.05), respectively, which were 0.68,0.81 nmol·L-1.6, respectively. Compared with the model group, the cAMP concentrations were significantly increased (P <0.05 or P <0.01) CAMP / cGMP increased (P <0.05 or P <0.01). The galactose-free, high-dose of Astragalus membranaceus and Amomum villosum had the same effect on cAMP concentration, but no significant difference was found. Conclusion: The volatile oil contained in six herbs can increase cAMP content, cAMP / cGMP and PDE content in gastric tissue, some of them also have similar effects on high-dose de-oiling oil group, The volatile oil contained was closely related to the effect of cold and warm in the cold. It was also found that the detoxifying oil extract of this six-flavor medicine also had a good effect on some test indexes, indicating that its efficacy was also related to the other components contained therein.