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目的 研究分析在不同级别脑胶质瘤细胞端粒酶活性的表达及端粒长度的变化。方法 采集 40例脑胶质瘤手术切除标本、 4例正常脑组织 ,通过半定量端粒重复序列扩增 (telomererepeatamplificationprotocol,TRAP) 银染方法检测端粒酶活性水平 ,应用人的端粒序列特异性探针32 P (CCC TAA) 3 进行Southern杂交检测脑胶质瘤细胞的端粒长度。结果 在 40例胶质瘤标本中的 33例(82 5 % )中均检出端粒酶活性 ,而在正常脑组织中无端粒酶活性的表达 ,不同级别胶质瘤之间端粒酶活性水平有明显差异 ;恶性胶质瘤细胞中端粒的长度明显比正常胶质细胞缩短 ,端粒的长度与端粒酶活性的水平有着显著的的负相关。结论 端粒酶活性可以作为脑胶质瘤的恶性标记之一 ,端粒的缩短可能是脑胶质瘤进展的重要因素 ,端粒的修复机制对于维持端粒的稳定性和肿瘤细胞的增殖潜能具有十分重要的意义
Objective To study the expression of telomerase activity and telomere length changes in glioma cells of different grades. Methods Forty cases of glioma were excised and four normal brain tissues were collected. Telomerase activity was detected by semi-quantitative telomere repeat amplification protocol (TRAP). The telomerase activity The probe 32 P (CCC TAA) 3 was used to detect telomere length of glioma cells by Southern blot. Results Telomerase activity was detected in 33 (82.5%) of 40 glioma specimens, but no telomerase activity in normal brain tissues. The telomerase activity The level of telomerase in malignant glioma cells was significantly shorter than that in normal glial cells, and the telomere length had a significant negative correlation with telomerase activity. Conclusion Telomerase activity may be one of the malignant markers of glioma. The shortening of telomere may be an important factor in the progress of glioma. The mechanism of telomere repair is crucial to the stability of telomere and the proliferation potential of tumor cells It is of great significance