论文部分内容阅读
目的 :研究乙酰胆碱 (ACh)受体在皮质酮 (CORT)对大鼠头端延髓腹外侧区 (RVLM)前交感神经元快速效应中的作用 ,探讨糖皮质激素在交感心血管活动调节中的非基因组机制。方法 :本研究采用细胞外记录和微电泳等方法观察CORT对氨基甲酸乙酯麻醉大鼠RVLM前交感神经元的作用 ,观察分别给予ACh受体拮抗剂阿托品 (ATR)、筒箭毒 (d TC)或六烃季铵 (C6)后CORT对RVLM前交感神经元的影响。结果 :在RVLM共记录到 33个前交感神经元 ,CORT能导致 2 5 (76 % )个前交感神经元快速兴奋 ,且具有剂量依赖性 ,余 8个前交感神经元没有反应 ;其中被CORT兴奋的 10个单位微电泳ATR后神经元的放电明显下降 ,但对CORT导致的兴奋作用没有明显的影响。分别向 7和 6个被CORT兴奋的前交感神经元微电泳d TC和C6后 ,单位放电没有变化 ,同时对CORT导致的兴奋作用无影响。结论 :CORT对RVLM前交感神经元具有快速的兴奋作用 ,这种作用可能并不通过ACh受体介导
AIM: To investigate the role of acetylcholine (ACh) receptor in the rapid response of corticosterone (CORT) to the presynaptic neurons in the rostral ventrolateral medulla (RVLM) of rats and to explore the role of glucocorticoid in the regulation of sympathetic cardiovascular activity Genome mechanism. Methods: The effect of CORT on RVLM presympathetic neurons was observed by extracellular recording and micro-electrophoresis. ATR, ACh receptor antagonist ATR, ) Or hexa-hydrocarbon quaternary ammonium (C6) after CORT on RVLM presympathetic neurons. RESULTS: A total of 33 presympathetic neurons were recorded in RVLM. CORT resulted in rapid excitement of 25 (76%) presympathetic neurons in a dose-dependent manner and no response to the other 8 presympathetic neurons. Among them, CORT Excited 10 units of micro-electrophoresis after ATR neuronal discharge decreased significantly, but the excitement caused by CORT no significant effect. There were no changes in unit discharges to 7 and 6 pre-sympathetic neuronal electrophoresis d TC and C6 excitated by CORT, respectively, and no effect on the excitatory effects caused by CORT. CONCLUSION: CORT has a rapid stimulatory effect on RVLM presympathetic neurons, and this effect may not be mediated by ACh receptors