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为评价ＣＤ４４分子过量表达在葡萄膜与结膜黑色素瘤发生发展过程中的作用，应用流式细胞术（ＦＣＭ）及细胞免疫荧光染色技术对葡萄膜恶性黑色素瘤（ＵＭＭ）、结膜色素痣（ＣＮ）及恶性黑色素瘤（ＣＭＭ）的ＣＤ４４Ｖ表达进行定量检测。结果表明：７例ＣＭＭ较５例ＣＮ的ＣＤ４４Ｖ表达量明显增加（Ｐ＜０．０５），７例ＣＭＭ及４０例ＵＭＭ的ＣＤ４４Ｖ表达阳性率分别为７１．４３％及６２．５％；ＵＭＭ中伴巩膜受侵犯者较不伴巩膜受侵犯者的ＣＤ４４Ｖ表达量明显增加（Ｐ＝０．０１０５）；ＵＭＭ的ＣＤ４４Ｖ表达量与增殖指数（ＰＩ）、Ｓ期细胞百分率（ＳＰＦ）呈显著负相关（Ｐ＜０．０１；Ｐ＜０．０５）。以上表明ＣＤ４４Ｖ过量表达与ＣＭＭ及ＵＭＭ的发生有关，并与ＵＭＭ局部浸润有关。
To evaluate the role of overexpression of CD44 in the development of uveal and conjunctival melanomas, flow cytometry (FCM) and immunofluorescence staining were applied to uveal malignant melanoma (UMM) and conjunctival pigmentary nevus (CN). Quantification of CD44V expression in malignant melanoma (CMM). The results showed that the expression of CD44V was significantly increased in 7 cases of CMM compared with 5 cases of CN (P<0.05). The positive rates of CD44V expression in 7 cases of CMM and 40 cases of UMM were 71.43% and 62.5%, respectively; The expression of CD44V in sclera-affected individuals was significantly higher than that in sclera-affected individuals (P=0.0105). The expression of CD44V in UMM was significantly negatively correlated with proliferation index (PI) and percentage of S-phase cells (SPF). P<0.01; P<0.05). The above indicates that the overexpression of CD44V is related to the occurrence of CMM and UMM, and related to the local infiltration of UMM.