皮肤是培养成纤维细胞最丰富又最容易获取的材料,其中又以包皮,特别是包皮的皮下疏松结缔组织为最好。因为它不但取材方便、操作简单、培养的成活率高,而且细胞生长快,可缩短培养周期,早出结果。材料与方法经外科手术取下包皮(如果仅为诊断用皮块,大小为2mm×4～5mm即 Skin is the most abundant and readily available material for culturing fibroblasts, with the foreskin, especially the subcutaneous loose connective tissue of the foreskin, being the best. Because it is not only easy access, simple operation, high survival rate of culture, and rapid cell growth, can shorten the incubation period, early results. Materials and methods Surgical removal of the foreskin (if only for the diagnosis of skin, the size of 2mm × 4 ~ 5mm