论文部分内容阅读
血清中的硫氰酸盐(s-SCN)、可的宁(s-COT)和呼出气中的一氧化碳(CO-ex)是判断吸烟状况的常用生物化学指标。为了把吸烟状况分类错误的可能性减少到最低限度,作者对同一样本中的常用生物化学指标进行了检测,并对其敏感度和特异性进行了评价。研究对象为泰勒马克县的145名年龄46～65岁的健康男性受试者。个人报告并经同事证实的吸烟状况表明,127人为吸烟者,7人为不吸烟者,11人是在1982～1989年停止吸烟的戒烟者。于1989年3月至1990年1月采集静脉血标本,在2h内离心处理,24h内将血清于-20℃冷冻保存。用Cobas Bio离心分析仪直接测定s-SCN含量,以>50μmol/L为判定吸烟者的s-SCN标准。用两倍抗体放射免疫测定法检测s-COT,以>71μg/L为判定
Serum thiocyanate (s-SCN), cortinol (s-COT), and carbon monoxide (CO-ex) in exhaled air are common biochemical indicators of smoking status. To minimize the possibility of misclassification of smoking status, the authors examined common biochemical indicators in the same sample and evaluated their sensitivity and specificity. The study population was 145 healthy male subjects aged 46 to 65 years in Telemark County. Personal reports and confirmed smoking status by colleagues showed that 127 were smokers, seven were non-smokers and 11 were smokers who stopped smoking between 1982 and 1989. Venous blood samples were collected from March 1989 to January 1990 and centrifuged within 2 hours. The serum was frozen at -20 ° C within 24 hours. The content of s-SCN was measured directly by Cobas Bio Centrifugal Analyzer, and the s-SCN standard of smokers was judged as> 50μmol / L. S-COT was detected by double antibody radioimmunoassay, which was judged as> 71μg / L