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目的:观察静脉注射清开灵、甲基强的松龙(MP)注射液及其联合用药对油酸型急性肺损伤(ALI)家兔的影响。方法:家兔随机分为11组:对照组,油酸组,治疗组分别包括清开灵和MP注射液以及清开灵+MP注射液小、中、大剂量组。iv油酸(0.05mL·kg-1)复制家兔ALI模型后,立即iv上述药液,观察给予油酸前及以后30,60,120min时的呼吸频率和幅度,于各时间点测定动脉血气含量;实验后处死家兔取肺组织,测定肺组织LDH,CAT和MDA的含量,及观察病理变化。结果:与油酸组比较,清开灵和MP注射液小、中、大剂量组的呼吸幅度显著升高、呼吸频率显著降低(P<0.05);治疗30min时,清开灵+MP注射液小、大剂量组的PaO2显著升高(P<0.05),120min时,清开灵、MP及清开灵+MP注射液小、中和大剂量组的PaCO2显著降低(P<0.05);清开灵、MP注射液中、大剂量组的LDH显著升高、CAT和MDA显著降低(P<0.05);清开灵+MP小、中剂量组可以减轻由油酸引起的病理改变。结论:清开灵+MP小剂量对油酸型肺损伤家兔的疗效优于同剂量下的单独用药;而清开灵或MP大剂量单独给药的疗效优于同剂量下的联合用药。
Objective: To observe the effects of intravenous injection of Qingkailing and methylprednisolone (MP) and their combination on oleic acid-induced acute lung injury (ALI) in rabbits. Methods: Rabbits were randomly divided into 11 groups: control group, oleic acid group, treatment group, including Qingkailing and MP injection and Qingkailing + MP injection small, medium and large dose groups. iv oleic acid (0.05mL · kg-1) rabbits ALI model immediately after iv injection of the above liquid to observe the oleic acid before and after 30,60,120 min respiration rate and amplitude at each time point determination of arterial blood gas content After the experiment, the rabbits were sacrificed to take the lung tissue, and the content of LDH, CAT and MDA in the lung tissue were measured, and the pathological changes were observed. Results: Compared with the oleic acid group, the respiratory rate of Qingkailing and MP injection groups were significantly increased (P <0.05), and the Qingkailing + MP injection group (P <0.05). The PaCO2 of small, medium and high dose groups of Qingkailing, MP and Qingkailing + MP injection significantly decreased at 120min (P <0.05) In Kailing and MP injection, the LDH of high dose group increased significantly and CAT and MDA decreased significantly (P <0.05). The small and medium dose Qingkailing + MP group could reduce the pathological changes caused by oleic acid. Conclusion: Qingkailing + MP low dose of oleic acid-induced lung injury in rabbits is better than the same dose of single drug; and Qingkailing or MP high dose alone is better than the same dose of combination therapy.