论文部分内容阅读
目的分析儿童端粒长度与血压水平以及血压偏高检出率之间的关联性,为有效预防儿童高血压提供参考。方法在2012年卫生行业科研专项基线数据库中,按照体质量指数(BMI)筛选湖南、天津、辽宁、上海4省市7~12岁儿童253名,按照BMI水平分为正常体重(81名)、超重和轻中度肥胖(85名)、重度肥胖(87名)3组,采用实时荧光定量PCR方法测定白细胞端粒长度,并计算对数转换的端粒长度。采用Pearson相关分析端粒长度与血压水平之间的关联性。结果采用美国国家高血压教育项目(NHBPEP)标准和中国高血压联盟(CHL)标准,253名7~12岁儿童的血压偏高检出率分别为15.81%和24.90%,男生分别为17.36%和28.93%,女生分别为14.39%和21.21%,男生均高于女生,差异均无统计学意义(P值均>0.05)。基于NHBPEP标准,血压偏高组儿童的端粒检测长度(TLT)和对数转换长度(Log-TLT)分别为1.07和0.02,与正常血压组儿童长度(分别为1.09和0.03)差异均无统计学意义(P值均>0.05);基于CHL标准,血压偏高组儿童的端粒长度TLT和Log-TLT分别为1.09和0.03,与正常血压组儿童长度(1.08和0.03)差异均无统计学意义(P值均>0.05)。男、女生呈现相同趋势,差异无统计学意义(P>0.05)。在BMI正常、超重和轻中度肥胖、重度肥胖3组学生中基本呈现相同趋势。结论儿童时期端粒长度与血压水平和血压偏高检出无明显的关联性,还需要进一步在成人人群中进行验证。
Objective To analyze the correlation between the telomere length of children and the blood pressure level and the high detection rate of high blood pressure so as to provide a reference for the effective prevention of hypertension in children. Methods A total of 253 children aged 7-12 years from 4 provinces and municipalities in Hunan, Tianjin, Liaoning and Shanghai were screened by body mass index (BMI) according to the scientific research baseline database of health industry in 2012. The subjects were divided into normal weight (81) Overweight and mild to moderate obesity (85), severe obesity (87) three groups, using real-time fluorescence quantitative PCR method to measure leukocyte telomere length, and calculate the logarithmically converted telomere length. Pearson correlation was used to analyze the association between telomere length and blood pressure level. Results The prevalence of high blood pressure in 253 children aged 7 to 12 years was 15.81% and 24.90% respectively, with male students being 17.36% and male students respectively using NHBPEP and CHL criteria 28.93%, girls were 14.39% and 21.21%, boys were higher than girls, the difference was not statistically significant (P> 0.05). Based on the NHBPEP criteria, TLT and Log-TLT in children with high blood pressure were 1.07 and 0.02, respectively, and were not statistically different from those in children with normal blood pressure (1.09 and 0.03, respectively) (P> 0.05). Based on the CHL standard, TLT and Log-TLT of telomere length in children with high blood pressure were 1.09 and 0.03, respectively. There was no significant difference between the two groups in children’s length (1.08 and 0.03) Significance (P> 0.05). Male and female showed the same trend, the difference was not statistically significant (P> 0.05). In BMI normal, overweight and mild to moderate obesity, severe obesity group of 3 students showed basically the same trend. Conclusion There was no significant correlation between telomere length and blood pressure level and high blood pressure in childhood, which should be further verified in the adult population.