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我们曾报道,单独的外周神经的C类纤维传入能在猫的体感皮层(SI)诱发一个特异的C类纤维皮层诱发电位(C-CEP),它可被镇痛药度冷丁和吗啡所抑制;电刺激大脑皮层前外侧回联合区(ALA)能对C-CEP产生明显的抑作用。本实验拟静脉注射阿片受体拮抗剂纳洛酮,观察对C-CEP和电刺激ALA抑制C-CEP作用的影响,以便探讨内源性阿片样物质在此过程中的可能作用。 实验方法 实验用猫10只,体重1.5～2.5公斤,雌雄不拘。手术在氯醛糖麻醉和三碘季铵酚制动下进行。切开头皮,开颅,暴露右侧大脑皮层后乙状回(SI区)和前外侧回联合区。分离左侧隐神经,结扎并剪断其外周端。在近中端分别放置刺激电极、银一氯化银阻断电极和记录电极,以便分别作电刺激隐神经、阻滞隐神经A类纤维传导和记录隐神经动作电位之用。电刺激ALA用两极同心圆电极。实验过程的其他处理步
We have reported that afferent C-fiber importation of peripheral nerves induces a specific C-cell-cortical evoked potential (C-CEP) in the cat’s somatosensory cortex (SI), which can be induced by analgesics Inhibition; electrical stimulation of the anterolateral conjunctiva (ALA) of the cerebral cortex had a significant inhibitory effect on C-CEP. In this study, intravenous injection of naloxone, an opioid receptor antagonist, was performed to observe the effect of C-CEP and C-CEP by electrical stimulation of ALA in order to explore the possible role of endogenous opioid in this process. Experimental method Experimental cat 10, weighing 1.5 to 2.5 kg, male or female. Surgery in the chloral hydrate anesthesia and triiodide quaternary ammonium phenol under the arrest. Cut the scalp, craniotomy, exposing the right cerebral cortex after the sigmoid (SI) and anterolateral back to the combined area. Separation of the left saphenous nerve, ligation and cutting its peripheral end. Stimulation electrodes, silver silver chloride blocker electrodes and recording electrodes were placed near the mid-end respectively for electrical stimulation of saphenous nerve, blockage of saphenous nerve fiber class A conduction and recording of saphenous nerve action potentials. Electrically stimulated ALA with bipolar concentric electrodes. Other processing steps of the experiment