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目的:探讨CD40基因多态性与云南汉族人群肺癌发生发展的相关性。方法:选取云南地区汉族人群肺癌Ⅰ+Ⅱ期患者151例、Ⅲ+Ⅳ期患者316例,同时选择云南地区汉族健康体检人群384例(对照组),采用Taq Man探针基因分型方法对两组被检者CD40基因中rs1883832(C>T)和rs4810485(G>T)2个多态性位点进行基因分型,观察等位基因、基因型及所构建的单倍型在肺癌Ⅰ+Ⅱ期患者、肺癌Ⅲ+Ⅳ期患者和对照组的分布频率。结果:rs4810485位点等位基因G和T在Ⅲ+Ⅳ期肺癌和对照组被检者的分布频率差异具有统计学意义(P=0.013);遗传模式的分析结果经年龄校正后,rs1883832位点在Dominant模式下CC基因型和CT~TT基因型在Ⅰ+Ⅱ期及Ⅲ+Ⅳ期肺癌患者分别与对照组的分布频率比较,差异具有统计学意义(P=0.011和0.008);rs4810485位点在Dominant模式下基因型GG和基因型GT~TTde分布频率比较亦具有统计学意义(P=0.008和0.001)。结论:CD40基因中多态性位点rs4810485位点等位基因G和基因型GG可能是肺癌发生的风险性因素,而rs1883832位点基因型CC可能是肺癌发生的风险因素。
Objective: To investigate the relationship between CD40 gene polymorphism and the occurrence and development of lung cancer in Yunnan Han population. Methods: One hundred and fifteen patients with stage Ⅰ + Ⅱ lung cancer and 316 patients with stage Ⅲ + Ⅳ disease were selected from Han nationality in Yunnan Province. 384 healthy controls (control group) were selected from Han nationality in Yunnan Province. Genotyping by Taq Man probe In the group of CD40 gene, rs1883832 (C> T) and rs4810485 (G> T) polymorphic loci were genotyped. The alleles, genotypes and haplotypes were determined in lung cancer Ⅰ + Ⅱ patients, lung cancer Ⅲ + Ⅳ patients and control group frequency of distribution. Results: The frequencies of alleles G and T of rs4810485 locus in patients with stage Ⅲ + Ⅳ lung cancer and the control group were significantly different (P = 0.013). The analysis of genetic patterns showed that age-adjusted rs1883832 locus The frequencies of CC genotypes and CT ~ TT genotypes in patients with stage Ⅰ + Ⅱ and stage Ⅲ + Ⅳ were significantly different from those in control group (P = 0.011 and 0.008) in Dominant mode; rs4810485 In Dominant mode, genotype GG and genotype GT ~ TTde distribution frequencies were also statistically significant (P = 0.008 and 0.001). Conclusion: Polymorphic site rs4810485 allele G and genotype GG of CD40 gene may be risk factors for lung cancer, while rs1883832 genotype CC may be a risk factor for lung cancer.