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应用接头连接PCR步行法,建立了分子水平鉴定不同转基因事件的方法和体系;并利用不同转基因事件外源基因与植物基因组DNA整合位点处核酸序列的差异,设计了不同转基因烟草事件的标签引物,成功地对几个转TMV-CP材料进行了鉴定。
The method and system for identification of different transgenic events at the molecular level were established by using the linker-connected PCR walking method. Using the difference of the nucleotide sequences at the integration sites of the different transgenic events and plant genomic DNA, tag primers of different transgenic tobacco events were designed Several successful TMV-CP materials were successfully identified.