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背景:临床工作发现多种抗生素可影响甚至加重重症肌无力已存在的神经肌肉接头处传递功能的障碍,使患者的肌无力症状恶化。目前,国内外在重症肌无力动物模型上进行抗生素对神经肌肉接头处传递功能影响的报道较少。随着新型抗生素的出现,有必要进一步探讨各类抗生素对神经肌肉接头处传递功能的影响。目的:探讨头孢菌素类、喹诺酮类和氨基糖甙类抗生素对重症肌无力神经肌肉接头处传递功能的影响,为临床安全、合理选用抗生素治疗重症肌无力提供实验依据。设计:以实验动物为研究对象,随机对照试验。单位:一所大学医院的感染科、神经内科和药剂科。材料:实验于2002-03/2003-01在华中科技大学同济医学院神经科学研究所完成。健康、雌性C57BL/6小鼠150只,随机分为正常组10只;重症肌无力组10只;生理盐水组10只;抗生素治疗组120只。抗生素治疗组分为庆大霉素组、依米替星组、环丙沙星组、氟罗沙星组、头孢呋新组和头孢他啶组,每组20只。干预:以丁氏双鳍电鳐的电器官的乙酰胆碱受体免疫3次建成EA重症肌无力模型。生理盐水组、各抗生素治疗组于末次免疫后第7天开始按10mg/kg体质量分别注射生理盐水和抗生素,连续注射14d;重症肌无力组和正常组不做任何处理。分别于末次免疫后第7天和抗生素治疗后第14天进行症状评分、
BACKGROUND: Clinical work has found that multiple antibiotics can affect or even exacerbate the dysfunction of the transfer of function at the pre-existing neuromuscular junction of myasthenia gravis and worsen the patient’s muscle weakness. At present, there are few reports on the influence of antibiotics on the transmission function of neuromuscular junction in animal models of myasthenia gravis at home and abroad. With the emergence of new antibiotics, it is necessary to further explore the impact of various antibiotics on neuromuscular junction transfer function. OBJECTIVE: To investigate the effect of cephalosporins, quinolones and aminoglycosides on the transmission of neuromuscular junction in myasthenia gravis and provide experimental basis for clinical safety and rational use of antibiotics in the treatment of myasthenia gravis. Design: The experimental animals as the research object, randomized controlled trials. Unit: Department of Infectious Diseases, Neurology and Pharmacy in a University Hospital. MATERIALS: Experiments were performed at Institute of Neuroscience, Tongji Medical College, Huazhong University of Science and Technology from March 2002 to January 2003. 150 healthy and female C57BL / 6 mice were randomly divided into normal group (n = 10), myasthenia gravis group (n = 10), saline group (n = 10) and antibiotic treatment group (n = 120). Antibiotic treatment group was gentamicin group, according to miltexine group, ciprofloxacin group, fleroxacin group, cefuroxime group and ceftazidime group, each group 20. INTERVENTION: EA-induced myasthenia gravis model was established by immunization of Acetylcholine receptors in electrical organs of Dorsal finned flies three times. In the saline group and the antibiotic treatment group, normal saline and antibiotics were respectively injected by 10 mg / kg body weight on the 7th day after the last immunization and injected continuously for 14 days. The patients with myasthenia gravis and the normal group were given no treatment. Symptoms were scored on the 7th day after the last immunization and on the 14th day after antibiotic treatment.