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目的:探讨人类白细胞抗原(HLA)-DQB1基因多态性与新疆维吾尔族人群结核病易感性的关联。方法:采用病例-对照的研究方法,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术对226例新疆维吾尔族肺结核病患者(肺结核病例组)和231例新疆维吾尔族健康对照者(健康对照组)进行HLA-DQB1基因分型,比较其等位基因频率(GF),并计算其比值比(OR)。结果:肺结核病例组中HLA-DQB1*0201基因频率显著高于健康对照组,两组的GF分别为40.13%、19.15%,差异有统计学意义(P<0.05);肺结核病例组中HLA-DQB1*0301/4基因频率显著低于健康对照组,两组的GF分别为6.16%、10.27%,但P值经过校正后无显著性差异(P>0.05)。结论:HLA-DQB1*0201等位基因与新疆维吾尔族人群结核病强相关,DQB1*0201可能是其易感基因。
Objective: To investigate the association between human leucocyte antigen (HLA) -DQB1 gene polymorphism and tuberculosis susceptibility in Xinjiang Uygur population. Methods: A case-control study was conducted in 226 Uighur tuberculosis patients (tuberculosis cases) and 231 Uighur healthy controls (Xinjiang Uyghur) using polymerase chain reaction-sequence specific primers (PCR-SSP) Healthy control group) HLA-DQB1 genotyping, compared the allele frequency (GF), and calculate the odds ratio (OR). Results: The frequency of HLA-DQB1 * 0201 gene in tuberculosis cases was significantly higher than that in healthy controls (GF, GF) was 40.13% and 19.15% respectively, with significant difference (P <0.05) * 0301/4 gene frequency was significantly lower than the healthy control group, GF was 6.16%, 10.27%, respectively, but the P value after correction was no significant difference (P> 0.05). Conclusion: The HLA-DQB1 * 0201 allele is strongly associated with tuberculosis in Xinjiang Uygur population. DQB1 * 0201 may be the predisposing gene.