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目的： 比较２ 种测定神经生长因子活性的最常用方法， 对活性测定条件进行了部分改进。方法： 鸡胚背根神经节组织培养法， ＰＣ１２ 细胞培养法。结果： 鸡胚背根神经节培养法灵敏度为５０ μｇ·Ｌ－１ 。ＰＣ１２ 细胞用于定性测定灵敏度为２０ μｇ·Ｌ－ １ 。ＰＣ１２ 细胞用于定量测定灵敏度可达１ μｇ·Ｌ－１ 。结论： 鸡胚背根神经节培养法实验条件要求简单， 易于开展， 但操作复杂， 干扰因素多， 一般用于定性测定。ＰＣ１２ 细胞培养法操作简单， 干扰因素少， 实验结果客观， 但实验条件要求高， 需要有可靠的细胞株和完备的细胞培养设备。
OBJECTIVE: To compare two of the most common methods for determining the activity of nerve growth factor (NGF), and to partially improve the activity assay conditions. Methods: Chick embryo dorsal root ganglion tissue culture method, PC12 cell culture method. Results: The sensitivity of chicken embryo dorsal root ganglion was 50 μg · L-1. The sensitivity of PC12 cells for qualitative determination was 20 μg · L -1. PC12 cells for quantitative determination of sensitivity up to 1 μg · L-1. CONCLUSION: The experimental conditions of chicken embryo dorsal root ganglion culture are simple and easy to carry out. However, the operation is complicated with many interference factors and is generally used for qualitative determination. PC12 cell culture method is simple, less interference factors, the experimental results objective, but demanding experimental conditions, the need for a reliable cell line and a complete cell culture equipment.