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研究目的探讨Zn、Se、Mn、Ni对胰岛β细胞损伤的保护作用及其机制，为糖尿病的治疗开辟新的途径。研究方法用Wistar大鼠进行体外及体内实验。胰岛细胞体外培养，设正常对照，加Zn孔、加Se孔、加Mn孔、加Ni孔，培养1h测各孔胰岛素含量。然后加入脉链菌素（STZ），再测胰岛素含量。加16．7mM葡萄糖做刺激试验，培养3天，取培养液测脂质过氧化物（LPO）。60只雄性大鼠随机分为6组，即Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组。Ⅱ～Ⅳ组腹腔内注射STZ55mg／kg，Ⅰ组仅注同量的构检酸缓冲液。Ⅰ、Ⅱ组喂基础饲料，Ⅲ～Ⅵ组在基础饲料中分别加入ZnCO3、Na2SeO3、MnCl2、NiCl2。72h后出现轻重不等的DM现象，继续饲养3周处死，取胰腺组织做病理检查，胰腺匀浆测血糖、胰岛素、LPO、SOD及谷胱甘肽过氧化物酶（GSH－PX）。结果体外实验结果：加Zn、Se、Mn、Ni培养的胰岛细胞，其胰岛素分泌量、葡萄糖刺激胰岛素分泌量，明显高于单加STZ，而LPO值明显低于单加STZ。体内实验结果：Ⅰ、Ⅲ～Ⅵ组血糖、LPO均明显低于Ⅱ组，胰岛素、SOD、GSH－PX均明显高于Ⅱ组。胰腺病理检查：STZ组大鼠胰腺均有炎症改变几乎见不到正常日细胞，而加Zn、Se、Mn、Ni组大鼠胰岛β细胞破坏较轻。结论Zn、Se、Mn、Ni均对胰岛β细胞有不同程度的保护作用，?
The purpose of this study was to explore the protective effects and mechanisms of Zn, Se, Mn and Ni on islet β cell injury and to explore new ways for the treatment of diabetes mellitus. Methods Wistar rats were used for in vitro and in vivo experiments. Islet cells cultured in vitro, set the normal control, plus Zn hole, add Se hole, add Mn hole, add Ni hole, measured 1h of each hole insulin content. Then add streptozotocin (STZ), measure the insulin content again. Add 16.7mM glucose stimulation test, cultured for 3 days, take culture medium to measure lipid peroxides (LPO). 60 male rats were randomly divided into 6 groups, namely Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ group. Group Ⅱ ~ Ⅳ intraperitoneal injection of STZ55mg / kg, Ⅰ group only injected the same amount of the structure of acid buffer. Groups Ⅰ and Ⅱ fed with basal diet, and groups Ⅲ and Ⅵ were treated with ZnCO3, Na2SeO3, MnCl2 and NiCl2.72h respectively, and then DM was observed in the basal diet. The animals were sacrificed after 3 weeks of sacrifice, and their pancreatic tissues were taken for pathological examination. Blood glucose, insulin, LPO, SOD and glutathione peroxidase (GSH-PX) were measured. Results The results of in vitro experiments: Insulin secretion and glucose-stimulated insulin secretion of cultured islet cells cultured with Zn, Se, Mn and Ni were significantly higher than that of STZ alone, while LPO was significantly lower than that of STZ alone. The results of in vivo experiments: The blood glucose and LPO in group Ⅰ, Ⅲ ~ Ⅵ were significantly lower than those in group Ⅱ, insulin, SOD, GSH-PX were significantly higher than those in group Ⅱ. Pancreatic pathological examination: There were almost no changes in inflammation in the pancreas of rats in STZ group, while the islet cells in Zn, Se, Mn and Ni groups were less damaged. Conclusion Zn, Se, Mn and Ni all have different degrees of protective effects on pancreatic β cells.