论文部分内容阅读
目的:探讨游离脂肪酸(FFA)作用下胰岛βTC3细胞双链RNA依赖性蛋白样内质网激酶(PERK)的表达以及利拉鲁肽(Lira)对其表达的干预作用。方法:以βTC3细胞为研究对象,分为对照组和FFA组(0.125,0.25,0.5及1 mmol/L)孵育24 h,Westernblot方法检测PERK的表达。然后,分为对照组,FFA组,和FFA+Lira组(0.5 mg/L和1 mg/L),Lira预孵育6 h后,1 mmol/L FFA继续孵育24 h,Western blot检测PERK的表达。结果:①不同浓度FFA孵育24 h后,与对照组相比,1 mmol/L FFA组PERK表达增加(P<0.05)。②与1 mmol/L FFA组相比,0.5mg/L Lira+1 mmol/L FFA和1 mg/L Lira+1 mmol/L FFA组PERK表达减少(P<0.05),两组之间有统计学差异(P<0.05)。结论:FFA作用能够上调βTC3细胞PERK的表达,而Lira在一定程度上逆转FFA水平异常导致的βTC3细胞PERK表达上调,减轻内质网应激反应。
Objective: To investigate the expression of double-stranded RNA-dependent endoplasmic reticulum kinase (PERK) in pancreatic βTC3 cells induced by free fatty acid (FFA) and the effect of liraglutide on its expression. METHODS: βTC3 cells were divided into control group and FFA group (0.125, 0.25, 0.5, and 1 mmol / L) for 24 h. PERK expression was detected by Western blot. Then, the cells were divided into control group, FFA group and FFA + Lira group (0.5 mg / L and 1 mg / L) for 6 h after pretreatment with Lira, and incubated with 1 mmol / L FFA for 24 h. PERK expression was detected by Western blot . Results: ① After 24 h incubation with different concentrations of FFA, PERK expression was increased in 1 mmol / L FFA group compared with control group (P <0.05). ② Compared with 1 mmol / L FFA group, the expression of PERK in 0.5 mg / L Lira + 1 mmol / L FFA and 1 mg / L Lira + 1 mmol / L FFA group decreased (P <0.05) Learning difference (P <0.05). CONCLUSION: FFA can up-regulate the expression of PERK in βTC3 cells. However, Lira can up-regulate the expression of PERK in βTC3 cells induced by abnormal FFA to a certain extent, and reduce the endoplasmic reticulum stress response.