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目的寻找并克隆子前期胎盘组织高表达基因蛋白磷酸酶2A催化亚基β(PP2ACβ),研究其mR-NA及蛋白水平的表达,探讨其在子前期中的作用。方法标本取自2004年5月至2004年12月天津中心妇产科医院重度子前期及正常孕妇各30例。应用荧光mRNA差异显示技术(FDD)发现并克隆子前期胎盘组织差异表达的基因PP2ACβ,进一步应用半定量RT-PCR及免疫组化技术分析差异表达基因PP2ACβmRNA及其蛋白水平PP2A的表达。结果通过FDD发现PP2ACβ基因在子前期胎盘组织中高表达,半定量RT-PCR证实PP2ACβmRNA在子前期胎盘组织中的表达(0.888±0.104)高于正常胎盘组织(0.692±0.099),差异有统计学意义(P<0.05),免疫组化验证其蛋白水平PP2A的表达(0.14±0.02)亦高于正常(0.12±0.02),差异有统计学意义(P<0.05)。结论FDD可有效用于筛选子前期胎盘组织差异表达基因;PP2A可能通过促进胎盘滋养层细胞的凋亡及阻碍血管生成而参与子前期的发病。
OBJECTIVE: To investigate the expression of PPARβ2 (PP2ACβ), a protein of high expression in the placenta of preeclampsia, and to investigate its role in pre-emodial stage. Methods The specimens were collected from 30 preeclampsia and normal pregnant women in Tianjin Central Obstetrics and Gynecology Hospital from May 2004 to December 2004. The differentially expressed PP2ACβ gene was detected by fluorescence differential display (FDD) and the differentially expressed PP2ACβ mRNA was analyzed by semi-quantitative RT-PCR and immunohistochemistry. Results The expression of PP2ACβ mRNA in placenta of preeclampsia was higher than that in normal placenta (0.888 ± 0.104) by semi-quantitative RT-PCR. The difference was statistically significant (P <0.05). The protein level of PP2A (0.14 ± 0.02) was also significantly higher than that of normal (0.12 ± 0.02) by immunohistochemistry. The difference was statistically significant (P <0.05). Conclusion FDD can be used to screen the differentially expressed genes of pre-placenta tissue of premature baby. PP2A may play an important role in the pre-emneal stage by promoting the apoptosis of placental trophoblast and inhibiting the angiogenesis.