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目的:研究复方地黄对老年小鼠的神经生长因子改变及形态学的影响,进一步探讨复方地黄对老年痴呆的作用机制。方法:将实验小鼠随机分为4组,实验组给予复方地黄混浊液每天2mL/只灌胃。治疗组给予安理申混悬液每天400mg/kg,空白对照组和模型对照组用等量生理盐水。3周后,各组小鼠进行行为学测试,应用免疫组化法检测脑组织神经生长因子改变及电镜观察形态学改变。结果:复方地黄能够改善SAM-P/8小鼠的学习和记忆能力,减少痴呆模型小鼠的包涵体数量,增加大脑皮层及海马区神经营养因子的含量,对SAM-P/8小鼠具有较好防治作用。结论:复方地黄能够明显改善老年痴呆小鼠的学习能力和蛋白表达。
Objective: To study the effect of compound rehmannia root on nerve growth factor changes and morphology of aged mice, and further explore the mechanism of compound rehmannia on senile dementia. Methods: The experimental mice were randomly divided into 4 groups. The experimental group was given 2 mL of compound diuretic turbid liquid per day. The treatment group was given 400 mg/kg Anritsu suspension daily, and the control group and the model control group were given the same amount of physiological saline. After 3 weeks, mice in each group were tested for behavior. Nerve growth factor changes in brain tissue and morphological changes under electron microscope were detected by immunohistochemistry. RESULTS: Compound rehmannia could improve the learning and memory ability of SAM-P/8 mice, reduce the number of inclusion bodies in dementia model mice, increase the content of neurotrophic factors in cerebral cortex and hippocampus, and have the effect on SAM-P/8 mice. Better control effect. Conclusion: Compound Rehmannia can significantly improve the learning ability and protein expression of senile dementia mice.