对２７种棉花基因型的花药在离体培养中的反应进行了比较，结果表明：所有的基因型在合适的培养条件下均能高频率地形成愈伤组织，但不同基因型间愈伤组织诱导率和诱导量不同；再生植株的花药比原始亲本的花药易诱导出愈伤组织；品种间杂种表现出部分优势，但不明显。本试验中，仅鲁棉６号和Ｓｉｏｋｒａ１－３两个品种诱导获得了胚性愈伤组织、胚状体和再生植株，且二者之间也存在着差别。从基因型在棉花花药培养中的效应、外界调控对棉花不同基因型花药培养效果的影响、棉花花药培养与体细胞培养基因型差异的比较等三个方面进行了讨论，提出了棉花花药培养愈伤组织诱导和植株再生是受两套遗传基因控制的遗传性状，并提出了棉花组织培养植株再生的阈值问题 The responses of 27 cotton genotypes to the anther culture in vitro were compared. The results showed that all the genotypes could form callus with high frequency under suitable culture conditions, but the callus The induction rate and induction amount were different. The anther of regenerated plants could induce callus more easily than the anthers of the original parents. The hybrids showed some advantages but not obvious. In this experiment, only embryogenic callus, embryoid body and regenerated plants were induced by two varieties of Lintan 6 and Siokra1-3, and there were also differences between the two. The effects of genotypes on cotton anther culture, the effect of external regulation on anther culture effects of different genotypes of cotton, the comparison of anther culture of cotton anthers and somatic cell culture genotypes were discussed. Wound tissue induction and plant regeneration are controlled by two sets of genetic genetic traits, and proposed the threshold of cotton tissue culture plant regeneration threshold