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目的:通过观察桑拿高温脱水和低氧恢复期运动时的白细胞热休克蛋白70(HSP70)、最大吸氧量(VO2max)、血乳酸(BLa)的变化,并与常温条件下相同运动负荷时相比较,探讨桑拿高温脱水和低氧刺激后恢复期运动对热休克蛋白表达和有氧能力的影响。方法:10名身体健康的体育系男性大学生,在三种不同环境下进行递增负荷运动:(1)桑拿前(常温条件,25℃,相对湿度65%);(2)桑拿后(进行桑拿浴之后,常温条件,25℃,相对湿度65%);(3)低氧后(低氧实施期之后,常温条件,25℃,相对湿度65%),分别测试递增负荷运动前安静时、运动后即刻、运动后3小时受试者白细胞HSP70表达、血液SOD活性和MDA含量及VO2max、血乳酸(BLa)和HR等生理机能指标。结果:(1)桑拿前安静时的HSP70(13701.87±5367.17)水平明显高于桑拿后(7517.57±1980.01,P<0.05)和低氧后(2850.79±2233.81,P<0.05);(2)桑拿后和低氧后运动后恢复期除MDA外,HSP70水平、SOD浓度低于桑拿前。(3)桑拿后和低氧后的VO2max及运动时间都较桑拿前略有增加。同时HRmax和BLa浓度有所下降,但无显著性差异(P>0.05)。以上结果表明,运动负荷可增加热休克蛋白70表达量,在桑拿后恢复期有可能具有“应激保护”作用。桑拿和低氧刺激后运动负荷的刺激,增加有氧能力,可能与热休克蛋白合成变化有关。
OBJECTIVE: To observe the changes of HSP70, VO2max and BLa in sauna during the period of high temperature dehydration and hypoxic recuperation exercise, and to compare the changes of HSP70, The effects of hyperthermia and hypoxia-stimulated convalescent exercise on heat shock protein expression and aerobic capacity were investigated. METHODS: Ten healthy male college males were subjected to an increasing load of exercise in three different environments: (1) before sauna (at room temperature, 25 ° C, 65% relative humidity); (2) after saunas (3) After hypoxia (hypoxia period, normal temperature, 25 ℃, relative humidity 65%), respectively, test the progressive load before exercise is quiet, after exercise The expression of HSP70, SOD activity, MDA content and VO2max, BLa and HR physiological indexes were measured immediately after exercise for 3 hours. Results: (1) The level of HSP70 (13701.87 ± 5367.17) after being quiet before sauna was significantly higher than that after sauna (7517.57 ± 1980.01, P <0.05) and after hypoxia (2850.79 ± 2233.81, P <0.05) After hypoxia and exercise recovery in addition to MDA, HSP70 levels, SOD concentration was lower than before sauna. (3) After the sauna and hypoxia after VO2max and exercise time slightly increased before the sauna. At the same time, the concentration of HRmax and BLa decreased, but no significant difference (P> 0.05). The above results show that exercise load can increase the expression of heat shock protein 70 in the recovery period after the sauna may have a “stress protection” effect. Stimulation of the sauna and exercise load after hypoxia increased the aerobic capacity, which may be related to the synthesis of heat shock protein.