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目的监测胸膜斑阳性接尘工人血清效应蛋白质,探讨胸膜斑发病机制及其与石棉肺的关系。方法将研究对象分为胸膜斑组、阴性对照组和正常对照组。各组人群静脉血应用双向电泳和质谱进行分离和鉴定,以灰度值差异1.5倍为基准获得差异表达蛋白质。结果胸膜斑组与阴性对照组和正常对照组比较,差异点分别有34和16个;阴性对照组与正常对照组比较,差异点有32个。效应蛋白质如补体因子、α1-抗胰蛋白酶等参与机体免疫、抗肿瘤等过程。部分效应蛋白质与石棉肺血清效应蛋白一致。结论长期接触石棉导致血清中某些蛋白质表达量改变,进而影响其生物学功能。胸膜斑和石棉肺有相同的靶效应蛋白,提示胸膜斑工人更易患石棉肺。
Objective To monitor serum effect proteins in workers with pleural plaques and explore the relationship between pleural plaque pathogenesis and asbestosis. Methods The subjects were divided into pleural plaque group, negative control group and normal control group. The venous blood of each group was separated and identified by two-dimensional electrophoresis and mass spectrometry, and differentially expressed proteins were obtained based on the difference of 1.5 times the gray value. Results There were 34 and 16 differences between the control group and the negative control group and the normal control group respectively. There were 32 differences between the negative control group and the normal control group. Effect of protein such as complement factor, α1-antitrypsin involved in immune, anti-tumor and other processes. Part of the effect of protein and asbestos lung serum effect protein. Conclusion Long-term exposure to asbestos causes changes in the expression of certain proteins in serum and further affects its biological function. Pleural plaque and asbestos lung have the same target-responsive protein, suggesting that pleural plaque workers are more susceptible to asbestosis.