To investigate the relationship between velocity structure and earthquake activity on the southeastern front of the Tibetan Plateau, we make use of continuous observations of seismic ambient noise data obtained at 55 broadband stations from the regional Yunnan Seismic Network. These data are used to compute Rayleigh wave Green’s Functions by cross-correlating between two stations, extracting phase velocity dispersion curves, and finally inverting to image Rayleigh wave phase velocity with periods between 5 and 34 s by ambient noise tomography. The results show significant lateral variations in crustal and uppermost mantle structures in the studied region. Phase velocity anomalies at short periods(5–12 s) are closely related to regional tectonic features such as sediment thickness and the depth of the crystalline basement. The Sichuan-Yunnan rhombic block, enclosed by the Honghe, Xiaojiang and Jianchuan faults, emerges as a large range of low-velocity anomalies at periods of 16–26 s, that inverts to high-velocity anomalies at periods of 30–34 s. The phase velocity variation in the vicinity of the Sichuan-Yunnan rhombic block suggests that the low-velocity anomaly area in the middle-lower crust may correspond to lower crustal channelized flow of the Tibetan Plateau. The spatial distribution of strong earthquakes since 1970 reveals that the Yunnan region is inhomogeneous and shows prominent characteristics of block motion. However, earthquakes mostly occur in the upper crust, with the exception of the middle-Yunnan block where earthquakes occur at the interface zone between high and low velocity as well as in the low-velocity zones, with magnitudes being generally less than 7. There are few earthquakes of magnitude 5 at the depths of 15–30 km, where gather earthquakes of magnitude 7 or higher ones which mainly occur in the interface zone between high and low velocities with others extending to the high-velocity abnormal zone. To investigate the relationship between velocity structure and earthquake activity on the southeastern front of the Tibetan Plateau, we make use of continuous observations of seismic ambient noise data obtained at 55 broadband stations from the regional Yunnan Seismic Network. These data are used to compute Rayleigh wave Green’s Functions by cross-correlating between two stations, extracting phase velocity dispersion curves, and finally inverting to image Rayleigh wave phase velocity with periods between 5 and 34 s by ambient noise tomography. The results show significant lateral variations in crustal and uppermost mantle structures in The studied region. Phase velocity anomalies at short periods (5-12 s) are closely related to regional tectonic features such as sediment thickness and the depth of the crystalline basement. The Sichuan-Yunnan rhombic block, enclosed by the Honghe, Xiaojiang and Jianchuan faults, emerges as a large range of low-velocity anomalies at periods of 16-26 s , that inverts to high-velocity anomalies at periods of 30-34 s. The phase velocity variation in the vicinity of the Sichuan-Yunnan rhombic block suggests that the low-velocity anomaly area in the middle-lower crust may correspond to lower crustal channelized flow of the Tibetan Plateau. The spatial distribution of strong earthquakes since 1970 reveals that the Yunnan region is inhomogeneous and shows prominent characteristics of block motion. However, earthquakes mostly occur in the upper crust, with the exception of the middle-Yunnan block where earthquakes occur at the interface zone between high and low velocity as well as in the low-velocity zones, with magnitudes being generally less than 7. There are few earthquakes of magnitude 5 at the depths of 15-30 km, where gather earthquakes of magnitude 7 or higher ones which mainly occur in the interface zone between high and low velocities with others extending to the high-velocity abnormal zone.