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目的探讨重组人(rh)IL-11、rhG-CSF联合应用对小鼠脾脏T淋巴细胞数量和功能的影响及其机制。方法应用流式细胞仪对细胞因子处理后的各组T淋巴细胞和亚群、共刺激分子CD28以及抑制性T淋巴细胞(CD3+CD4-CD8-、CD8+CD28-、CD4+CD25+)的数量进行测定;应用MTT法检测各组T淋巴细胞的增殖能力、混合淋巴细胞反应;测定细胞内IL-4、IFN-γ的分泌情况。结果经rhG-CSF单独及rhIL-11、rhG-CSF联合处理淋巴细胞总数和T细胞亚群与对照组比较均下降(P<0.05),而联合处理组CD3+、CD4+、CD8+细胞下降较明显,与其它各组比较差异有统计学意义(P<0.01),CD4+/CD8+细胞的比值联合处理组亦低于其它各组;G-CSF组及联合处理组CD3+CD28+细胞比例与对照组比较下降(P<0.01),CD4+CD28+、CD8+CD28+细胞联合处理组虽较其它各组低,但各组间差异无统计学意义;CD3+CD4-CD8-和CD4+CD25+抑制性T淋巴细胞各组间均无明显差异,而联合处理组CD8+CD28-抑制性T淋巴细胞较其它各组增加(P<0.05);细胞因子动员后各组T淋巴细胞增殖能力、供鼠对异种抗原的反应能力均降低(P<0.05),而联合处理组比其它各组下降更明显;联合处理组、G-CSF组与对照组比较细胞内细胞因子IFN-γ水平下降、IL-4水平升高,联合处理组与G-CSF组比较差异无统计学意义,但IFN-γ/IL-4的比值联合组低于其它各组(P<0.05)。结论rhIL-11与rhG-CSF体内应用后可产生协同作用,通过对T淋巴细胞数量和功能的影响来诱导机体免疫耐受。
Objective To investigate the effects of rhIL-11 and rhG-CSF combined with rhG-CSF on the number and function of spleen T lymphocytes in mice and its mechanism. Methods The number of T lymphocytes and subsets, costimulatory molecules CD28 and inhibitory T lymphocytes (CD3 + CD4-CD8-, CD8 + CD28-, CD4 + CD25 +) in each group after cytokine treatment were analyzed by flow cytometry The proliferation of T lymphocytes in each group was measured by MTT assay and the lymphocyte reaction was mixed. The secretion of IL-4 and IFN-γ in the cells was measured. Results Compared with the control group, the total number of lymphocytes and T lymphocyte subsets of rhG-CSF treated with rhG-CSF alone, rhIL-11 and rhG-CSF decreased significantly (P <0.05), while the combination of rhG-CSF and rhG- (P <0.01). The ratio of CD4 + / CD8 + cells in the combined treatment group was also lower than that in other groups. The proportion of CD3 + CD28 + cells in the G-CSF group and the combined treatment group was lower than that in the control group (P <0.01). The combined treatment of CD4 + CD28 + and CD8 + CD28 + cells was lower than that of other groups, but there was no significant difference between the groups. CD3 + CD4-CD8- and CD4 + CD25 + inhibitory T lymphocytes There was no significant difference between the two groups, but the combined treatment group CD8 + CD28-suppressor T lymphocytes increased compared with other groups (P <0.05); after the mobilization of cytokines T lymphocyte proliferation ability, for the rat response to heterogeneous antigens (P <0.05), while the combined treatment group decreased more significantly than other groups; the combined treatment group, G-CSF group compared with the control group cytokines IFN-γ levels decreased, IL-4 levels increased, There was no significant difference between the combined treatment group and G-CSF group, but the ratio of IFN-γ / IL-4 in combination group was lower than that in other groups P <0.05). Conclusion rhIL-11 and rhG-CSF can produce a synergistic effect after in vivo application, and induce immune tolerance by affecting the number and function of T lymphocytes.