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目的 建立伊犁贝母中无紫外吸收的活性异甾体生物碱西贝素和西贝素苷的HPLC测定方法。方法HPLC 蒸发光散射检测器法。结果 西贝素苷和西贝素的线性范围分别为 48 72 - 194 88μg·mL-1和 6 0 72 -16 1 92 μg·mL-1;RSD日内 0 7%和 0 9% ,日间 1 0 %和 1 5 % ;低、中、高浓度回收率分别为 95 8% ,96 9% ,96 0 %和 97 1% ,96 7% ,96 8% ;最低检测限分别为 1 5 3和 2 43μg·mL-1。结论 本法快速、简便、灵敏和分离度好 ,适用于伊贝母中活性生物碱的测定。
Objective To establish an HPLC method for the determination of the active isosteroidal alkaloids, sibescocin and siberverin, in UV-free absorption of Ipilia frondosa. Methods HPLC Evaporative Light Scattering Detector method. Results The linear ranges of escin and siberin were 48 72 - 194 88 μg·mL -1 and 6 0 72 -16 1 92 μg·mL -1 , respectively; RSD was 0 7% and 0 9% during the day, and 1 0 % and 15 % ; low, medium and high concentrations of recovery were 95 8%, 96 9%, 96 0 % and 97 1%, 96 7%, 96 8%; the detection limit was 1 5 3 and 2 43 μg·mL-1. Conclusion This method is rapid, simple, sensitive and has good resolution. It is suitable for the determination of active alkaloids in I.