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目的了解湖南省感染性腹泻哨点医院儿童诺如病毒的感染状况及基因型别。方法 2012年1月-2014年12月采集湖南省哨点医院腹泻患儿的粪便标本,应用诺如病毒特异性引物进行扩增,选择扩增阳性产物进行基因测序和进化分析。结果 936份粪便标本RT-PCR检测诺如病毒核酸,阳性100份,阳性率10.68%。对40份诺如病毒核酸阳性标本进行基因序列测定与分析,38份为GⅡ型,其中31份为GⅡ.4型。在31株GⅡ.4型中,Sydney 2012和GⅡ.4 Den Haag 2006b各栓出14株。结论 2012-2014年湖南省哨点医院儿童腹泻病例中诺如病毒的感染率较高,GⅡ.4是优势株,其中GⅡ.4 sydney 2012和Den Haag 2006b是主要型别。
Objective To understand the infection status and genotypes of children Norovirus in sentinel hospital of infectious diarrhea in Hunan Province. Methods From January 2012 to December 2014, stool samples from children with diarrhea in sentinel hospital of Hunan Province were collected and amplified with Norovirus specific primers. The positive products were selected for gene sequencing and evolution analysis. Results 936 stool specimens were detected by RT-PCR for Norovirus nucleic acid. The positive rate was 10.68%. Forty-eight Norovirus-positive samples were sequenced and analyzed, of which 38 were of type GII and 31 of them were of type GII.4. Among 31 GⅡ.4 strains, 14 strains were knocked out by Sydney 2012 and GⅡ.4 Den Haag 2006b. CONCLUSION: The prevalence of norovirus in children with sentinel hospital in Hunan Province from 2012 to 2014 was higher than that of GDN-4, and GⅡ.4 sydney 2012 and Den Haag 2006b were the major types.