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黑莓种子中含有丰富的维生素E,采用超声波提取,结合反相高效液相色谱-紫外检测法对黑莓种子中维生素E的含量进行测定,确定体积比50∶50的乙腈∶甲醇作为流动相,以维生素E质量浓度X(μg/mL)对峰面积Y(mAU.min)进行线性回归,得回归方程Y=0.860 5X+0.596 6,相关系数为0.999 1,该方法维生素E的最低检出限为2 ng。通过本法对黑莓种子油稀释后样品进行测定,平均质量浓度1.820μg/mL,RSD=0.55%。得出黑莓种子中维生素E的含量为18.2 mg/100 g。VE平均加标回收率为99.88%,RSD<2%。结果证明,该方法具有简便、高效、结果准确、重复性好的特点。
Blackberry seeds are rich in vitamin E, the use of ultrasonic extraction, combined with reverse-phase high performance liquid chromatography - UV detection of blackberry seeds in the determination of vitamin E content to determine the volume ratio of 50:50 acetonitrile: methanol as the mobile phase to The regression equation Y = 0.860 5X + 0.596 6, the correlation coefficient was 0.999 1. The minimum detectable limit of vitamin E in this method was 2 ng. By this method of determination of the blackberry seed oil diluted samples, the average mass concentration of 1.820μg / mL, RSD = 0.55%. The result showed that the content of vitamin E in blackberry seeds was 18.2 mg / 100 g. The average recovery of VE was 99.88% with RSD <2%. The results show that this method is simple, efficient, accurate and reproducible.