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目的探讨生化分析仪速率法检测结果出现负值的原因及其消除干扰措施。方法应用全自动生化分析仪设置380 nm和410 nm两种副波长,同时对日常标本中溶血和脂浊标本进行ALT、AST、HBDH、BUN、CO_2五项生化实验进行对比检测。结果副波长设置为380hm时,Hb>2.5g/L干扰显著,设置为410 nm时,Hb>5.0 g/L有显著干扰,两组干扰值间的差异具有有统计学意义(P<0.05);TG>9.8 mmol/L对ALT、AST结果产生干扰,TG>14.6 mmol/L对HBDH、BuN、CO_2结果产生干扰,TG两组干扰值间差异无统计学意义(P>0.05);随着溶血、脂浊的加深干扰逐渐加大。脂浊血清经稀释后或10 000 r/min离心后的下清液血清标本检测结果相近。结论对于溶血标本进行以上五项检测时。副波长设置为410 nm可以较好地消除轻度溶血的干扰,对于脂浊标本通过样本稀释或提高离心速度,在一定程度上可以消除脂浊的干扰。
Objective To investigate the negative value of biochemical analyzer rate test and its countermeasures. Methods Two kinds of secondary wavelengths of 380 nm and 410 nm were set up by automatic biochemical analyzer. The biochemical experiments of ALT, AST, HBDH, BUN and CO_2 were performed in hemolytic and lipophilic samples in routine samples. Results The interference of Hb> 2.5g / L was significant when the secondary wavelength was set to 380hm. When Hb> 5.0g / L was set to 410nm, there was a significant interference. The difference between the two groups had statistical significance (P <0.05) ; TG> 9.8 mmol / L had a negative effect on ALT and AST, while TG> 14.6 mmol / L had interference on the results of HBDH, BuN and CO_2. There was no significant difference in TG between the two groups (P> 0.05) Hemolysis, lipophilic deepening interference gradually increased. Lipid serum after dilution or 10 000 r / min after centrifugation of serum samples were similar serum test results. Conclusion Hemolysis specimens for the above five tests. The setting of the sub-wavelength to 410 nm can eliminate the mild hemolysis interference. For the turbid samples, the sample dilution or increase of the centrifugal speed can eliminate the turbid interference to a certain extent.