本文分别研究了培养基中氮源组成与浓度，以及Ｋ＋、Ｃａ２＋、Ｍｇ２＋、ＰＯ４３－和ＳＯ４２－对茶愈伤组织生长和儿茶素累积含量的影响。结果表明，全硝态氮对于愈伤组织儿茶素形成最为有利；而ＮＯ３－／ＮＨ４＋比为７：３时愈伤组织生长最快；总氮浓度为１５ｍｍｏｌ／Ｌ时愈伤组织生长量最高；提高氮浓度则导致儿茶素含量急剧下降。添加Ｋ＋促进愈伤组织生长并使儿茶素含量降低；而１ｍｍｏｌ／Ｌ的Ｃａ２＋对儿茶素形成最为有利。在其余的离子中，ＰＯ４３－对愈伤组织生长影响较大，而ＳＯ４２－对儿茶素形成影响较大，Ｍｇ２＋浓度对两者均无显著影响。所得结果为采用两步法体系培养茶树细胞、系统研究儿茶素类物质的形成奠定了基础。 In this paper, the composition and concentration of nitrogen source in culture medium and the effects of K +, Ca2 +, Mg2 +, PO43- and SO42- on the callus growth and the content of catechin were studied respectively. The results showed that all-nitrate nitrogen was the most favorable for the formation of catechin in callus, while the callus grew fastest when NO3- / NH4 + ratio was 7: 3. The highest callus growth was found when the total nitrogen concentration was 15mmol / L ; Increasing nitrogen concentration led to a sharp drop in catechin content. The addition of K + promoted callus growth and decreased the content of catechin, while Ca (superscript 2 +) at 1 mmol / L was most favorable to catechin formation. Of the remaining ions, PO43- has a greater effect on callus growth, while SO42- has a greater effect on the formation of catechin, with no significant effect of Mg2 + concentration on both. The results obtained for the cultivation of tea tree cells by two-step system, the systematic study of the formation of catechins laid the foundation.