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目的:了解bFGF联合骨髓干细胞动员剂促进大鼠缺血后肢血管新生的作用。方法:将48只大鼠的左侧股动脉与其分支切断,构建大鼠后肢缺血模型,并按随机数字表法分成四组,每组12只,其中A组将重组人bFGF蛋白注入大鼠缺血后肢肌内;B组将重组人粒细胞集落刺激因子(rhG-CSF)注入大鼠皮下;C组大鼠予以bFGF联合rhG-CSF;D组予以均等的生理盐水。治疗持续4周后予以每组大鼠腹主动脉造影,进而观察大鼠侧支血管的具体形成状况,予以内收肌以及腓肠肌肌组织病理切片检查,借助图像分析系统,统计各组大鼠血管密度。行免疫组化检测,分析大鼠血管内皮生长因子阳性表述血管数。结果:在大鼠侧支循环血管条数、血管内皮生长因子阳性表述血管数以及血管密度上,C组>A组>B组>D组,差异均有统计学意义(P<0.05)。结论:对于促大鼠缺血后肢血管新生而言,bFGF蛋白与骨髓干细胞动员剂疗效显著,均可改善大鼠肢体的缺血状态,但骨髓干细胞动员剂联合bFGF蛋白改善大鼠缺血肢体更为明显。
Objective: To investigate the effect of bFGF combined with bone marrow stem cell mobilization agent on angiogenesis in ischemic hindlimb in rats. Methods: The left femoral artery of 48 rats were cut off from the branches of the femoral artery to establish the hind limb ischemia model of rats. The rats were divided into four groups (n = 12) by random number table. The rats in group A were injected with recombinant human bFGF protein In group B, recombinant human granulocyte colony-stimulating factor (rhG-CSF) was subcutaneously injected into rats. Group B received bFGF combined with rhG-CSF. Group D received equal volume of saline. After 4 weeks of treatment, abdominal aorta of each group was given angiography, and then the formation of collateral vessels in rats was observed. The adductor muscle and gastrocnemius muscular tissue histopathological examination were performed. With the help of image analysis system, density. Immunohistochemical detection of vascular endothelial growth factor-positive rat blood vessels. Results: There were significant differences in collateral circulation vascular number, vascular endothelial growth factor positive expression vascularity and vascular density in group C> group A> group B> D (P <0.05). CONCLUSION: The bFGF protein and bone marrow stem cell mobilization agent are effective in promoting rat ischemic hindlimb angiogenesis, both of which can improve the ischemic status of rat limbs. However, the combination of bFGF mobilizing agent and bFGF protein can improve the ischemic limbs of rats Obvious.