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１材料和方法１．１材料我院１９９７／１９９８大肠癌手术标本存档蜡块４０例，复习ＨＥ切片，选择癌与癌旁交界处蜡块其中高分化腺癌５例，中分化腺癌３２例，低分化腺癌３例，取切端粘膜１０例作正常对照；同时选取淋巴结转移癌蜡块１０例．以上标本均用中性福尔马林固定、
1 Materials and methods 1.1 Materials Our hospital 1997/1998 colorectal cancer surgical specimen archives 40 cases of wax, review HE slices, select the cancer and paracancerous borders among the well-differentiated adenocarcinoma in 5 cases, moderately differentiated adenocarcinoma in 32 cases 3. In 3 cases of poorly differentiated adenocarcinoma, 10 cases of cut end mucosa were taken as normal controls; 10 cases of lymph node metastasis cancer wax were also selected. The above specimens were fixed with neutral formalin.