Aim: To investigate the thrombolytic efficacy, ideal dosage and administration of native recombinant staphylokinase (r-SAK). Methods: Forty New Zealand rab-bits were randomly assigned into the control, r-SAK low-dose, medial-dose, high-dose, single bolus, allied therapy, recombinant streptokinase (r-SK) and uroki-nase (UK) groups. The fight femoral artery thrombosis models were made by balloon injury, and 120 min after the injury, the thrombolytic agents were infused through the rabbits’ parallel-ear vein. Results: (1) 2 h after balloon injury, the pulse pressures of the fight femoral arteries reduced to 0 or less than 10% of that of left femoral arteries in all groups; (2) after thrombolytic therapy, the pulse pressures in some of the femoral arteries markedly enhanced to more than 50% of that of left femoral arteries; (3) the reopening rates in the r-SAK medial and high-dose groups were significantly higher than that of the control. The reopening rate of the same dose native r-SAK was significant higher than that of UK and r-SK; (4) the patency score of the right femoral arteries tended to be better in the r-SAK medial and high-dose groups than that of the low-dose group, and the time to reopening in the allied therapy group tended to be shorter. Conclusion: (1) r-SAK has a definite thrombolytic effect on the femoral artery thrombus of rabbits; (2) single bolus is an effective manner of r-SAK therapy, and r-SAK allied therapy with hepafin may shorten the time to recanalization; (3) the efficacy of the same dose native r-SAK was superior to that of r-SK and UK.