论文部分内容阅读
目的研究胃腺癌组织中CD133蛋白表达及其临床意义。方法应用免疫组织化学染色方法检测99例胃癌患者手术切除的原发灶及正常胃黏膜组织中CD133蛋白的表达,分析其与临床病理特征和预后的关系。结果 29例(29.29%)患者肿瘤组织中CD133蛋白表达阳性,正常胃黏膜组织中均为阴性(P=0.000)。肿瘤直径>5 cm者CD133蛋白表达阳性率显著高于≤5 cm者(P=0.041);CD133蛋白表达与TNM分期有关(P=0.044);有淋巴结转移(P=0.017)、淋巴管浸润(P=0.000)和血管浸润(P=0.000)者,CD133蛋白表达显著增高。logistic回归分析显示:肿瘤浸润深度(P=0.011)、淋巴结转移(P=0.043)和TNM分期(P=0.049)分别是CD133蛋白表达阳性的独立危险因素。CD133蛋白表达阳性患者的术后生存时间短于表达阴性患者(P=0.046)。Cox比例风险回归模型分析显示,有淋巴结转移(P=0.042)、TNM分期(P=0.046)及CD133蛋白表达阳性(P=0.046)分别是胃癌患者预后的独立危险因素。结论胃癌组织中CD133蛋白的表达与胃癌的发展、转移及预后密切相关。
Objective To study the expression of CD133 protein in gastric adenocarcinoma and its clinical significance. Methods Immunohistochemical staining was used to detect the expression of CD133 protein in primary resection and normal gastric mucosa in 99 gastric cancer patients. The relationship between the expression of CD133 protein and clinicopathological features and prognosis was analyzed. Results Totally 29 cases (29.29%) had positive expression of CD133 protein in tumor tissues and negative in normal gastric mucosa tissues (P = 0.000). The positive rate of CD133 protein in tumor with diameter> 5 cm was significantly higher than that in ≤ 5 cm (P = 0.041). CD133 protein expression was related to TNM stage (P = 0.044), lymph node metastasis (P = 0.017) P = 0.000) and vascular invasion (P = 0.000), CD133 protein expression was significantly higher. Logistic regression analysis showed that the depth of tumor invasion (P = 0.011), lymph node metastasis (P = 0.043) and TNM stage (P = 0.049) were independent risk factors of CD133 protein positive. Patients with CD133 positive expression had shorter survival time than those with negative expression (P = 0.046). Cox proportional hazards regression model analysis showed that lymph node metastasis (P = 0.042), TNM stage (P = 0.046) and CD133 protein positive (P = 0.046) were independent risk factors for prognosis of gastric cancer patients. Conclusion The expression of CD133 protein in gastric cancer is closely related to the development, metastasis and prognosis of gastric cancer.