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This study demonstrated the potential of single chamber up-flow membrane-less microbial fuel cell (UFML-MFC) in wastewater treatment and power generation.The purpose of this study was to evaluate and enhance the performance under different operational conditions which affect the chemical oxygen demand (COD) reduction and power generation,including the increase of KCl concentration (MFC1) and COD concentration (MFC2).The results showed that the increase of KCI concentration is an important factor in up-flow membrane-less MFC to enhance the ease of electron transfer from anode to cathode.The increase of COD concentration in MFC2 could led to the drop of voltage output due to the prompt of biofilm growth in MFC2 cathode which could increase the intal resistance.It also showed that the COD concentration is a vital issue in up-flow membrane-less MFC.Despite the COD reduction was up to 96％,the power output remained constrained.