论文部分内容阅读
相互作用蛋白的发现与研究有助于精确地掌握蛋白质相互作用网络中各个蛋白之间的联系以及这些联系产生的生理功能。表达克隆筛选技术是经典的相互作用蛋白筛选技术之一,其基本原理是选择可能还有目的基因的文库,根据实验需要设计不同的筛选方式,经过反复筛选富集目的基因,再通过大规模测序获得目的基因序列。本文着重对报告基因筛选、黏附筛选、血清学筛选等不同筛选方法进行概括总结,为更好地利用这一方法进行相互作用蛋白筛选提供参考。
Discovery and research of interacting proteins help to pinpoint the precise relationships between the proteins in a protein-protein interaction network and the physiological functions that these connections produce. The expression cloning screening technology is one of the classical screening technologies for interacting proteins. The basic principle is to select libraries of possible target genes. Different screening methods are designed according to the experiments. After repeated screening and enrichment of the target genes, large-scale sequencing Obtain the target gene sequence. This article focuses on the screening of reporter genes, adhesion screening, serological screening and other screening methods summarized, in order to make better use of this method to interact protein screening provide a reference.