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探讨尖吻蝮蛇毒类凝血酶（ＤＡＴＬＥ）的促凝作用特点及影响其药效的有关因素。方法测定ＤＡＴＬＥ诱导的体外全血浆及吸咐血浆凝固时间，不同实验条件对ＤＡＴＬＥ及凝血酶诱导体外全血浆凝固时间（ＴＴ）、白陶土部分凝血活酶时间（ＫＰＴＴ）和凝血酶原时间（ＰＴ）的影响。结果在体外，ＤＡＴＬＥ能直接使人全血浆及吸咐血浆凝固，并呈剂量相关性，该促凝作用不受ＡＴ－Ⅲ和肝素的影响，Ｎａ＋离子抑制，而Ｃａ２＋离子能增强其促凝作用；ＤＡＴＬＥ促凝作用的最适ｐＨ值在５．０～８．５之间；ＤＡＴＬＥ在体内能增强内源性和外源性凝血作用，在０．０５～０．１ｍｇ／ｋｇ的促凝剂量下，ＤＡＴＬＥ略使血浆纤原含量减少。结论ＤＡＴＬＥ为一类凝血酶，在一定剂量范围内，主要表现为促凝作用，有不同于凝血酶的特点。
To investigate the procoagulant effect of DATLE and related factors that affect its efficacy. Methods DATLE-induced in vitro whole plasma and aspiration plasma coagulation time were measured. The effects of different experimental conditions on the in vitro total thromboplastin time (TT), KPTT, and PT )Impact. Results In vitro, DATLE directly coagulated human whole plasma and aspirated plasma in a dose-dependent manner. The procoagulant effect was not affected by AT-Ⅲ and heparin, while Na + ions were inhibited. However, Ca2 + ions enhanced its procoagulant effect ; DATLE procoagulant optimum pH value between 5.0 to 8.5; DATLE can enhance endogenous and exogenous coagulation in the body, at a coagulant dose of 0.05 ~ 0.1mg / kg Under, DATLE slightly reduced plasma fibrinogen content. Conclusion DATLE is a kind of thrombin, which is mainly procoagulant in a certain dose range, which is different from that of thrombin.