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目的建立过敏性鼻炎颗粒中挥发油的毛细管气相色谱指纹图谱,为过敏性鼻炎颗粒的质量控制提供新方法。方法建立毛细管气相色谱法,采用氢火焰检测器及质谱进行检测分析。HP-5 Phenyl Methyl Siloxane毛细管色谱柱(30 m×0.32mm,0.25μm);检测器温度250℃;进样口温度200℃;柱温采取程序升温,初始温度50℃,以3℃/min升温至140℃,保持2 min,再以10℃/min升温至200℃,保持2 min。结果建立了过敏性鼻炎颗粒中挥发油的毛细管气相指纹图谱方法,确定了过敏性鼻炎颗粒中12个特征峰,并采用GC-MS对主要色谱峰进行了初步定性。10批成品指纹图谱相似度在0.90以上。结论建立的毛细管气相色谱指纹图谱有较好的精密度、重复性和稳定性,适用于过敏性鼻炎颗粒的质量控制。
Objective To establish a capillary gas chromatographic fingerprint of volatile oil in allergic rhinitis granules, which provides a new method for the quality control of allergic rhinitis granules. Methods The capillary gas chromatography was established. The detection was performed by hydrogen flame detector and mass spectrometry. HP-5 Phenyl Methyl Siloxane capillary column (30 m × 0.32 mm, 0.25 μm); detector temperature of 250 ° C .; inlet temperature of 200 ° C .; temperature programmed temperature rise of 50 ° C at 3 ° C / min To 140 ℃ for 2 min, and then heated to 200 ℃ at 10 ℃ / min for 2 min. Results A capillary GC fingerprinting method was established for the volatile oil in allergic rhinitis granules. Twelve characteristic peaks of the allergic rhinitis granules were identified, and the main chromatographic peaks were characterized by GC-MS. 10 batches of fingerprints similarity of 0.90 or more. Conclusion The established capillary GC fingerprinting has good precision, repeatability and stability and is suitable for the quality control of allergic rhinitis granules.