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背景与目的:了解四川省彝族人群中HIV_1辅助受体CX3CR1基因多态性在正常人和HIV_1感染者中的分布特点,探讨此辅助受体多态性对HIV感染的影响。材料与方法:从202份外周血中提取基因组DNA(正常人115份,HIV_1感染者87份)。用PCR_限制性片段长度多态性(PCR_RFLP)技术检测V249I和T280M两种变体,检测结果用行列表χ2检验法进行统计学分析。结果:在检测的115例正常人样品中,249I和280M等位基因频率分别为8.3%和5.7%;HIV感染者中,两种等位基因频率分别为7.5%和5.7%。249I和280M间存在明显的连锁关系。正常人和感染者的两种等位基因频率的差异无统计学意义(P>0.05)。结论:所获得的四川彝族人群HIV_1辅助受体CX3CR1基因多态性资料有助于进一步分析四川彝族人群HIV感染和艾滋病病程的影响因素。
BACKGROUND & AIM: To investigate the distribution of CX3CR1 gene polymorphism of HIV-1 co-receptor in normal people and HIV-1 infected persons in Yi population of Sichuan province and investigate the influence of this co-receptor polymorphism on HIV infection. Materials and Methods: Genomic DNA was extracted from 202 peripheral blood samples (115 normal subjects and 87 HIV-1 infected subjects). Two variants, V249I and T280M, were detected by PCR-restriction fragment length polymorphism (PCR_RFLP). The test results were statistically analyzed with the chi-square test. Results: The frequency of 249I and 280M alleles was 8.3% and 5.7% respectively in 115 normal samples tested. Among the HIV-infected patients, the frequencies of the two alleles were 7.5% and 5.7% respectively. There is a clear linkage between 249I and 280M. There was no significant difference in the frequencies of the two alleles between normal subjects and those infected (P> 0.05). Conclusion: The data of CX3CR1 gene polymorphism of HIV_1 co-receptor obtained from Sichuan Yi ethnic population is helpful to further analyze the influencing factors of the HIV infection and AIDS duration in Yi population in Sichuan province.